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http://www.addgene.org/100839

Species: Synthetic
Genetic Insert: GCaMP6m
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 ( p2817) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100839 Copy   

•   Source: Addgene

http://www.addgene.org/100834

Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (P3435) from the Penn Vector Core. GCaMP6f was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100834 Copy   

•   Source: Addgene

http://www.addgene.org/100799

Species: Synthetic
Genetic Insert: mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_100799 Copy   

•   Source: Addgene

http://www.addgene.org/100833

Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 ( p2819) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100833 Copy   

•   Source: Addgene

http://www.addgene.org/100845

Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (p2821) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100845 Copy   

•   Source: Addgene

http://www.addgene.org/100848

Species: Synthetic
Genetic Insert: jRCaMP1a
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100848 Copy   

•   Source: Addgene

http://www.addgene.org/100857

Species: Synthetic
Genetic Insert: jRGECO1b
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.NES-jRGECO1b.WPRE.SV40 (p3846) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100857 Copy   

•   Source: Addgene

http://www.addgene.org/100853

Species: Synthetic
Genetic Insert: jRGECO1a
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40(p3848) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100853 Copy   

•   Source: Addgene

http://www.addgene.org/100850

Species: Synthetic
Genetic Insert: jRCaMP1b
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (p3851) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100850 Copy   

•   Source: Addgene

http://www.addgene.org/100851

Species: Synthetic
Genetic Insert: jRCaMP1b
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid was previously available as pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (p3852)) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Proper citation: RRID:Addgene_100851 Copy   

•   Source: Addgene

http://www.addgene.org/101039

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:8506; Vector Backbone:eSpCas9(1.1); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_101039 Copy   

•   Source: Addgene

http://www.addgene.org/101218

Species: Other
Genetic Insert: SpCas9 variant N692A/M694A/Q695A/H698A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.

Proper citation: RRID:Addgene_101218 Copy   

•   Source: Addgene

http://www.addgene.org/101209

Species: Other
Genetic Insert: SpCas9 variant N497A/R661A/Q695A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.

Proper citation: RRID:Addgene_101209 Copy   

•   Source: Addgene

http://www.addgene.org/101361

Species: Other
Genetic Insert: Channelrhodopsin-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4606; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: ChR originally from P.Hegemann, HU-Berlin Germany tDimer originally from R-Tsien USD USA

Proper citation: RRID:Addgene_101361 Copy   

•   Source: Addgene

http://www.addgene.org/100054

Species: Other
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core PV1959

Proper citation: RRID:Addgene_100054 Copy   

•   Source: Addgene

http://www.addgene.org/100547

Species: Other
Genetic Insert: N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
Vector Backbone Description: Backbone Size:7064; Vector Backbone:pEF1a-FB-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_100547 Copy   

•   Source: Addgene

http://www.addgene.org/12402

Species:
Genetic Insert: 135(T2AG3)
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pSP73; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_12402 Copy   

•   Source: Addgene

http://www.addgene.org/12401

Species:
Genetic Insert: Sty11
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pSP73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert used as probe for telomere blots

Proper citation: RRID:Addgene_12401 Copy   

•   Source: Addgene

http://www.addgene.org/12456

Species:
Genetic Insert: TCF/LEF binding sites
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4871; Vector Backbone:pTA-Luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site). Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.

Proper citation: RRID:Addgene_12456 Copy   

•   Source: Addgene

http://www.addgene.org/12452

Species: Homo sapiens
Genetic Insert: EFP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Digestion by KpnI and XhoI should give a 5.4 kb fragment and a 2.4 kb fragment. Compared to GenBank ID NP_005073.2, the EFP insert in this plasmid appears to contain a P385L mutation, whereas when compared to GenBank ID AB384815.1 the EFP insert appears to contain G89V and A185T mutations. These apparent mutations may represent valid polymorphisms and reflect the sequence of the original human cDNA clone. The plasmid is exactly as described in the associated publication.

Proper citation: RRID:Addgene_12452 Copy   

•   Source: Addgene