Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pQnl_tet Resource Report Resource Website |
RRID:Addgene_191355 | PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette | Synthetic | Erythromycin | PMID:36427328 | Backbone Marker:Tolonen Lab; Backbone Size:5471; Vector Backbone:pQmod2E-GG (Addgene 191345); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 | ||
|
pATmin-GG Resource Report Resource Website |
RRID:Addgene_191352 | Plac-RFP (IPTG-inducible red fluorescent protein) | Other | Erythromycin | PMID:36427328 | Backbone Marker:PMID 19775243; Backbone Size:6972; Vector Backbone:pQexp; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 | ||
|
pEC3079 (p7INT-Pveg_lacI) Resource Report Resource Website |
RRID:Addgene_218505 | lacI | Other | Erythromycin | PMID:38911550 | This plasmid enables the constitutive expression of LacI in S. pyogenes. It can be used for inducible gene expression in combination with the Plac promoter variants described in the associated publication. Resource information: The Pveg promoter originates from the Bacillus subtilis PY79 genome. The lacI repressor is derived from pPEPY-PF6_lacI (Plasmid #85589) and originally codon-optimised for Streptococcus pneumoniae. However, codon usage is quite similar between S. pyogenes and S. pneumoniae. p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the file linked below to obtain a well annotated map of the plasmid. | Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-08-01 01:07:00 | 0 | |
|
pEC3135 (pERASE) Resource Report Resource Website |
RRID:Addgene_220306 | Erythromycin | PMID:38911550 | This plasmid allows to obtain scarless gene deletions in S. pyogenes and potentially other Gram-positive bacteria. The multiple cloning site (EcoRI, XbaI, SpeI, PstI) contains a Plac_mrfp cassette, allowing for red-white screening of clones in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white on LB agar plates. See the associated publication for more information. Resource Information: The pUC origin for replication in E. coli was taken from pUC19 (Plasmid #50005). The erythromycin resistance cassette was taken from p7INT. The MCS containing the mrfp cassette was taken from pSpy0C4. The coding sequence for the counterselection marker pheS* was taken from the native pheS coding sequence found in S. pyogenes SF370. This sequence was adapted to avoid recombination events of the plasmid at the phis locus due to high DNA sequence homology. Two mutations were introduced into the adapted pheS sequence to facilitate incorporation of the toxic phenylalanine analogue PCPA (4-chlor-DL-phenylalanine) into proteins, leading to bacterial cell death. p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the detailed plasmid map using the file linked below. | Backbone Size:4457; Vector Backbone:pERASE; Vector Types:Synthetic Biology; Bacterial Resistance:Erythromycin | 2024-08-01 01:07:15 | 0 | |||
|
pLPR3 Resource Report Resource Website |
RRID:Addgene_209986 | resistance cassette (ermBL) | Other | Erythromycin | PMID:39254046 | Vector Backbone:pLP5; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-12-13 04:06:39 | 0 | ||
|
pLPR1 Resource Report Resource Website |
RRID:Addgene_209979 | resistance cassette (ermBL) | Other | Erythromycin | PMID:39254046 | Vector Backbone:pLP2; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-12-13 04:06:39 | 0 | ||
|
pGBSedit Sham Resource Report Resource Website |
RRID:Addgene_223200 | N/A | Erythromycin | PMID: | Vector Backbone:pJC005.em; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | 2024-12-20 04:08:28 | 0 | |||
|
pGBScrispri Sham Resource Report Resource Website |
RRID:Addgene_223201 | N/A | Erythromycin | PMID: | Vector Backbone:pJC005.em; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | 2024-12-20 04:08:28 | 0 | |||
|
pMSP3535 Resource Report Resource Website 1+ mentions |
RRID:Addgene_46886 | Erythromycin | PMID:10964628 | This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3535 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Note: Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with the PnisA promoter. The depositing laboratory recommends adding of a Stop codon to the 5' end of inserted sequences to avoid unintentional gene fusions. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequencew which includes a mutation in RepE- F229L. This however is thought to not affect plasmid function. | Backbone Size:8353; Vector Backbone:pMSP3535; Vector Types:Bacterial Expression, Other, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin | 2025-01-09 04:10:12 | 3 | |||
|
pTlpA_mCherry_YafQ/DinJ Resource Report Resource Website |
RRID:Addgene_229148 | mCherry, DinJ/YafQ | Other | Erythromycin | PMID:36722614 | Addgene observed a ~1.4k base pair insertion downstream of the T7 terminator. This discrepancy does not affect plasmid function. | Backbone Marker:Addgene ; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2025-02-05 01:09:12 | 0 | |
|
pTec_mCherry Resource Report Resource Website |
RRID:Addgene_229145 | mCherry | Other | Erythromycin | PMID:38326819 | Backbone Marker:Addgene ; Backbone Size:2800; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | No | 2025-01-30 04:08:24 | 0 | |
|
pL2Cas9 Resource Report Resource Website |
RRID:Addgene_98841 | tracr/Cas9 | Erythromycin | PMID:28324650 | Note from the paper of O'Sullivan, and Klaenhammer: We found that BHI (Difco Laboratories, Detroit, MI, USA) was an excellent selection medium for ErR in E. coli. Unlike LB medium, BHI plates containing 150µg Er/ml afforded a clean selection, with no background colonies appearing even upon prolonged incubaction of one week or more. O'Sullivan, D.J., T.R. Klaenhammer. 1993. High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. Gene 137:227-231. | Backbone Size:6419; Vector Backbone:pTRKL2; Vector Types:; Bacterial Resistance:Erythromycin | 2023-04-01 01:09:12 | 0 | ||
|
pMG36E Resource Report Resource Website |
RRID:Addgene_133853 | Erythromycin | PMID:2495760 | Backbone Size:1800; Vector Backbone:pWV01; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:18 | 0 | ||||
|
pFCiKm7 Resource Report Resource Website |
RRID:Addgene_167521 | CinRAM-KmR | Synthetic | Erythromycin | PMID:34125913 | Vector Backbone:pFCiKm7; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Erythromycin | 2023-04-01 01:04:10 | 0 | ||
|
pJC005.em Resource Report Resource Website |
RRID:Addgene_182738 | Erythromycin | PMID:35107356 | Backbone Size:11031; Vector Backbone:pJC005.em; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | 2023-04-01 01:04:54 | 0 | ||||
|
pQexp Resource Report Resource Website |
RRID:Addgene_25820 | pQexp | Erythromycin | PMID:19775243 | Addgene sequence has a small insertion and small deletion compared to author sequence. These differences are in regions of the plasmid backbone that were not modified and should not affect function of this plasmid. | Backbone Size:6559; Vector Backbone:pAT19; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:05:42 | 0 | ||
|
EE-SP!gIII Resource Report Resource Website |
RRID:Addgene_48667 | Cas9 | Other | Erythromycin | PMID:24076762 | Vector Backbone:colE1-erm; Vector Types:CRISPR; Bacterial Resistance:Erythromycin | 2023-04-01 01:06:42 | 0 | ||
|
pLp_3050sNuc Resource Report Resource Website |
RRID:Addgene_122030 | signal peptide Lp_3050 | Other | Erythromycin | PMID:19744343 | Plasmid for heterologous secretion of proteins in Lactobacillus plantarum and L. sakei | Backbone Size:5600; Vector Backbone:spp-based expression vector (pSIP401); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:01:33 | 0 | |
|
pSIP403 Resource Report Resource Website |
RRID:Addgene_122028 | gusA | Erythromycin | PMID:16000734 | Backbone Size:5400; Vector Backbone:spp-based expression vector (pSIP401); Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-04-01 01:01:33 | 0 | |||
|
pQlox71F Resource Report Resource Website |
RRID:Addgene_135655 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Use in combination with pQlox66F (addgene 135657) or pQlox 66R (addgene 135658), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the FDI Lab - SciCrunch.org Resources search. From here you can search through a compilation of resources used by FDI Lab - SciCrunch.org and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that FDI Lab - SciCrunch.org has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on FDI Lab - SciCrunch.org then you can log in from here to get additional features in FDI Lab - SciCrunch.org such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into FDI Lab - SciCrunch.org you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
