Species: Other
Genetic Insert: attL1-ccdB-attL2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167671 Copy
Species:
Genetic Insert: C terminal VSV tag
Vector Backbone Description: Backbone Size:2852; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, Other, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26181 Copy
Species:
Genetic Insert: C terminal HA tag
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, Other, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26174 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Med21 aa1-132 for N-terminal tagging
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, Other, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Note that this plasmid must be grown in both Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_26183 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmcherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Please note: the orientation of the MCS within this plasmid places mCherry on the c-terminus of your gene of interest.
Proper citation: RRID:Addgene_31907 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12096; Vector Backbone:pMDC32; Vector Types:Other, Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_32078 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12767; Vector Backbone:pMDC32; Vector Types:Other, Gateway destination binary vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_32077 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Amaxa; Backbone Size:2800; Vector Backbone:pmaxGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_37631 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4492; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Other, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Replicative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29463 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4445; Vector Backbone:pMV361; Vector Types:Bacterial Expression, Other, Mycobacteria expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Integrative mycobacterial destination vector for recombination cloning.
Please note that there are multiple discrepancies between Addgene's quality control sequence and the depositor's theoretical sequence. The Hmama lab assures us that these differences should not affect plasmid function.
Proper citation: RRID:Addgene_29462 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7142; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Generic promoter-less destination vector
Proper citation: RRID:Addgene_29496 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:13844; Vector Backbone:pDW1714; Vector Types:Yeast Expression, Other, reporter; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Cloning nuclease targets into the BglII/SpeI sites of pCP5 removes the CcdB gene, thus allows negative selection against undigested backbone in common E. coli strains such as DH5-alpha.
Proper citation: RRID:Addgene_35397 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Other, GATEWAY entry vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Other, Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Other, GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51774 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2051; Vector Backbone:pENTR1A; Vector Types:Other, Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme. It serves to create intermediate supermodules with plant resistance cassettes.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 358-368bp
BsaI site #2 = 1796-1806bp
Proper citation: RRID:Addgene_48865 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Messing Lab, PMID 1905257; Backbone Size:3772; Vector Backbone:pOK1/2; Vector Types:Other, Gateway cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_48942 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5993; Vector Backbone:pDE43-MCK; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_49523 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:Other, plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
References:
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54382 Copy
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