Species: Other
Genetic Insert: ctxA
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Kaper, J. B., J. G. Morris, Jr., and M. Nishibuchi. 1988. DNA probes for pathogenic Vibrio species, p. 65-77. In F. C. Tenover (ed.), DNA probes for infectious disease. CRC Press, Inc., Boca, Raton, Fla.
Proper citation: RRID:Addgene_63690 Copy
Species: Homo sapiens
Genetic Insert: ICAM-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This clone will direct the expression of functional human ICAM-2 in a variety of cell types. This sequence has been deposited in Genbank with accession number X15606. Also see ref: Nature 329, 840-842 (1987)
Proper citation: RRID:Addgene_8634 Copy
Species: Homo sapiens
Genetic Insert: ICAM-1
Vector Backbone Description: Backbone Marker:BCCM/LMBP; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989)
Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison.
Proper citation: RRID:Addgene_8632 Copy
Species: Homo sapiens
Genetic Insert: MAC-1 alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This clone will direct the expression of functional Mac-1 upon cotransfection with the beta2 subunit in cos cells. This sequence has been deposited in Genbank with accession number J03925. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).
Proper citation: RRID:Addgene_8631 Copy
Species: Synthetic
Genetic Insert: tetA
Vector Backbone Description: Backbone Size:3101; Vector Backbone:mUAVbla; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_149379 Copy
Species:
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:5218; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_170985 Copy
Species:
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_170983 Copy
Species: Synthetic
Genetic Insert: OK-HEF s57a ED
Vector Backbone Description: Backbone Marker:-; Backbone Size:3500; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_175018 Copy
Species: Synthetic
Genetic Insert: OK-HEFwtED
Vector Backbone Description: Backbone Marker:-; Backbone Size:35; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_175017 Copy
Species: E.coli
Genetic Insert: rpsL tetA
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2100; Vector Backbone:pMOD4; Vector Types:Other, Recombineering; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_19184 Copy
Species:
Genetic Insert: Hemagglutinin A/MI/45/15 H1N1
Vector Backbone Description: Backbone Marker:-; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Plasmid may run as a dimer. Please try testing multiple colonies to isolate the monomer.
Proper citation: RRID:Addgene_158741 Copy
Species: Other
Genetic Insert: Neuraminidase A/NL/109/03
Vector Backbone Description: Backbone Marker:-; Backbone Size:4800; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_158793 Copy
Species:
Genetic Insert: Hemagglutinin A/TX/50/12 H3
Vector Backbone Description: Backbone Marker:-; Backbone Size:5400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_158739 Copy
Species:
Genetic Insert: H5_Vietnam_1203_04
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCD5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_182546 Copy
Species: Synthetic
Genetic Insert: GFP+
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5788; Vector Backbone:pTU1-A - EcoFlex (Moore et al, 2016 - PubMed PMID: 27096716); Vector Types:Bacterial Expression, Synthetic Biology, Other, E. coli and Bacillus shuttle vector; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_107577 Copy
Species: Synthetic
Genetic Insert: T7A1, O1, O-, Lambda t1 in lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170476 Copy
Species: Synthetic
Genetic Insert: T7A1, O-, O1, Lambda t1 in modified lacZ fragment
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: LacI operators separated by 400 base pairs.
Proper citation: RRID:Addgene_170474 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6393; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_201001 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6696; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200997 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21899 Copy
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