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http://www.addgene.org/102844

Species:
Genetic Insert: STAgR Insert gRNAScaffold_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_102844 Copy   

•   Source: Addgene

http://www.addgene.org/102845

Species:
Genetic Insert: STAgR Insert SAM_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_102845 Copy   

•   Source: Addgene

http://www.addgene.org/102842

Species:
Genetic Insert: gRNAScaffold_h7SKpromoter
Vector Backbone Description: Vector Backbone:pcDNA1; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_102842 Copy   

•   Source: Addgene

http://www.addgene.org/102855

Species: Synthetic
Genetic Insert: dSpCas9, pdDronpa1.2
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pPB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102855 Copy   

•   Source: Addgene

http://www.addgene.org/102949

Species: Mus musculus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Size:4141; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_102949 Copy   

•   Source: Addgene

http://www.addgene.org/102945

Species: Gallus gallus
Genetic Insert: SIINFEKL
Vector Backbone Description: Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint.

Proper citation: RRID:Addgene_102945 Copy   

•   Source: Addgene

http://www.addgene.org/102957

Species: Homo sapiens
Genetic Insert: MR1A cDNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3908; Vector Backbone:pCR2.1-TOPO; Vector Types:Other, Cloning; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102957 Copy   

•   Source: Addgene

http://www.addgene.org/102959

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2026; Vector Backbone:pRH144; Vector Types:Other, mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline
References:
Comments: Artificial DNA for plasmid pMin1 GenBank: Z50148.1

Proper citation: RRID:Addgene_102959 Copy   

•   Source: Addgene

http://www.addgene.org/103001

Species: Other
Genetic Insert: PfSSB_W166C
Vector Backbone Description: Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233.

Proper citation: RRID:Addgene_103001 Copy   

•   Source: Addgene

http://www.addgene.org/103000

Species: Other
Genetic Insert: PfSSB
Vector Backbone Description: Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233.

Proper citation: RRID:Addgene_103000 Copy   

•   Source: Addgene

http://www.addgene.org/102951

Species: Saccharomyces cerevisiae
Genetic Insert: APEX2-NES
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102951 Copy   

•   Source: Addgene

http://www.addgene.org/103007

Species: Danio rerio
Genetic Insert: myelin basic protein
Vector Backbone Description: Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103007 Copy   

•   Source: Addgene

http://www.addgene.org/103061

Species: Homo sapiens
Genetic Insert: integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (#52961); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Derived from pX330-U6-Chimeric_BB-CBh-hSpCas9

Proper citation: RRID:Addgene_103061 Copy   

•   Source: Addgene

http://www.addgene.org/103055

Species: Other
Genetic Insert: Ebolavirus minigenome
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103055 Copy   

•   Source: Addgene

http://www.addgene.org/103057

Species: Synthetic
Genetic Insert: TIMER-bac
Vector Backbone Description: Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance.

Proper citation: RRID:Addgene_103057 Copy   

•   Source: Addgene

http://www.addgene.org/103052

Species: Other
Genetic Insert: L gene of Ebola virus
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103052 Copy   

•   Source: Addgene

http://www.addgene.org/103051

Species: Other
Genetic Insert: VP30 gene of Ebola virus
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103051 Copy   

•   Source: Addgene

http://www.addgene.org/103053

Species: Other
Genetic Insert: VP35
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103053 Copy   

•   Source: Addgene

http://www.addgene.org/103063

Species: Other
Genetic Insert: Colicin E1 and E1 immunity protein
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene.

Proper citation: RRID:Addgene_103063 Copy   

•   Source: Addgene

http://www.addgene.org/103065

Species: Other
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter.

Proper citation: RRID:Addgene_103065 Copy   

•   Source: Addgene