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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
STAGR_gRNAScaffold_mU6
 
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1+ mentions
RRID:Addgene_102844 STAgR Insert gRNAScaffold_mU6 Kanamycin PMID:29702666 Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin 2022-04-22 03:15:20 1
STAGR_SAMScaffold_mU6
 
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RRID:Addgene_102845 STAgR Insert SAM_mU6 Kanamycin PMID:29702666 Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin 2022-04-22 03:15:21 0
STAGR_gRNAScaffold_h7SK
 
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RRID:Addgene_102842 gRNAScaffold_h7SKpromoter Kanamycin PMID:29702666 Vector Backbone:pcDNA1; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin 2022-04-22 03:15:20 0
VPR-ps-dSpCas9
 
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RRID:Addgene_102855 dSpCas9, pdDronpa1.2 Synthetic Ampicillin PMID:28938067 Backbone Marker:System Biosciences; Vector Backbone:pPB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin 2022-04-22 03:15:21 0
pCMV-5U-Venus-PSD-95-3U
 
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RRID:Addgene_102949 PSD-95 Mus musculus Kanamycin PMID:25948262 Backbone Size:4141; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:15:22 0
PresentER-SIINFEKL (mCherry)
 
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RRID:Addgene_102945 SIINFEKL Gallus gallus Ampicillin PMID:30499773 Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin OVA257-264 2022-04-22 03:15:22 0
pHMR1A
 
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RRID:Addgene_102957 MR1A cDNA Homo sapiens Ampicillin PMID:24832684 Backbone Marker:Invitrogen; Backbone Size:3908; Vector Backbone:pCR2.1-TOPO; Vector Types:Other, Cloning; Bacterial Resistance:Ampicillin Stop codon has been removed by HindIII site 2022-04-22 03:15:23 0
pMin1
 
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RRID:Addgene_102959 Tetracycline PMID:8566811 Artificial DNA for plasmid pMin1 GenBank: Z50148.1 Backbone Size:2026; Vector Backbone:pRH144; Vector Types:Other, mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline 2022-04-22 03:15:23 0
pET22b_PfSSB_W166C
 
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RRID:Addgene_103001 PfSSB_W166C Other Ampicillin PMID:29466468 Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C93A, W166C 2022-04-22 03:15:24 0
pET22b_PfSSB
 
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RRID:Addgene_103000 PfSSB Other Ampicillin PMID:29466468 The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
pJH124
 
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RRID:Addgene_102951 APEX2-NES Saccharomyces cerevisiae Ampicillin PMID:27274088 Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:23 0
pZL1-ZF - myelin basic protein a (mbpa)
 
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RRID:Addgene_103007 myelin basic protein Danio rerio Ampicillin PMID:12112375 Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
GESTALT_pX330-v1
 
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RRID:Addgene_103061 integration of Cas9 with guide targeting GESTALT barcodes V1 to V5 Homo sapiens Ampicillin PMID:27229144 Derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 Vector Backbone:lentiCRISPR v2 (#52961); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2022-04-22 03:15:25 0
pCAGGS_3E5E_luciferase
 
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RRID:Addgene_103055 Ebolavirus minigenome Other Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
pSC101_TIMER
 
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RRID:Addgene_103057 TIMER-bac Synthetic Kanamycin PMID:25126781 TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin mutations: S4T and S179T 2022-04-22 03:15:25 0
pCAGGS_L_EBOV
 
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RRID:Addgene_103052 L gene of Ebola virus Other Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
pCAGGS_VP30_EBOV
 
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RRID:Addgene_103051 VP30 gene of Ebola virus Other Ampicillin PMID:28807685 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
pCAGGS_HA-VP35_EBOV
 
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RRID:Addgene_103053 VP35 Other Ampicillin PMID:27252530 Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
pKSJ331
 
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RRID:Addgene_103063 Colicin E1 and E1 immunity protein Other Ampicillin PMID:19029376 Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:25 0
pKSJ376
 
Resource Report
Resource Website
RRID:Addgene_103065 colicin E1 immunity protein Other Ampicillin PMID:27795317 The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter. Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:25 0

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