Searching the RRID Resource Information Network
Species: Synthetic
Genetic Insert: Sr22 Schomburgk
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102833 Copy
Species: Synthetic
Genetic Insert: Sr22 Schomburgk
Vector Backbone Description: Vector Backbone:pVecBARII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_102835 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002 EQ
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102830 Copy
Species: Synthetic
Genetic Insert: SpyTag002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102831 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCAP05; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Tetracycline
References:
Comments: pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
Proper citation: RRID:Addgene_102920 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.
Proper citation: RRID:Addgene_102939 Copy
Species: Other
Genetic Insert: pknG
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: Addgene's quality control sequencing has identified a frameshift mutation near the C-terminus of the pET-15b backbone-resident Lac-I ORF. However, the depositing lab states that this mutation does not affect PknG protein expression.
Proper citation: RRID:Addgene_102814 Copy
Species: Other
Genetic Insert: OCT4-gRNAs-1-5-PGK-puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102893 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Bornkamm , et al. PMID 16147984; Vector Backbone:prTS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102934 Copy
Species: Homo sapiens
Genetic Insert: OMKSL-gRNAs-PGK-Puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102894 Copy
Species: Homo sapiens
Genetic Insert: EEA-5g-OSK2M2L1-gRNAs-PGK-Puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102909 Copy
Species: Other
Genetic Insert: OSK2M2L1-gRNAs-PGK-puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102902 Copy
Species: Other
Genetic Insert: EEA-guide1-PGK-Puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102904 Copy
Species:
Genetic Insert: Mouse Wnt5a
Vector Backbone Description: Backbone Size:4085; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Other, zebrafish expression; Xenopus expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102860 Copy
Species: Synthetic
Genetic Insert: ABE7.9
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102918 Copy
Species: Synthetic
Genetic Insert: ABE7.10
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102919 Copy
Species: Synthetic
Genetic Insert: ABE6.3
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102916 Copy
Species: Rattus norvegicus
Genetic Insert: KIF1A-shRNA
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:5415; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: The pmTurquoise KIF1A shRNA vector was constructed by restriction site removal of eGFP from the commercially available pSuper vector (Oligoengine) and insertion of the cDNA coding for pmTurquoise (Addgene plasmid #36202).
The reference for the pSuper Vector (Oligoengine) is T.R. Brummelkamp, R. Bernards, and R Agami, Science 296, 550 (2002).
Proper citation: RRID:Addgene_102849 Copy
Species:
Genetic Insert: gRNAScaffold_hU6promoter
Vector Backbone Description: Backbone Size:3250; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102843 Copy
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