Searching the RRID Resource Information Network
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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p11d-tRPA-32Asp8 Resource Report Resource Website |
RRID:Addgene_102617 | RPA1 | Homo sapiens | Ampicillin | PMID:12819197 | Note: The RPA2 gene in this plasmid is a variant that also contains a T88I mutation. | Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |
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p2lox-cAMPr R739A Resource Report Resource Website |
RRID:Addgene_102618 | cAMPr | Ampicillin | PMID:29511120 | Backbone Size:3300; Vector Backbone:P2lox; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin | R739A | 2022-04-22 03:15:17 | 0 | ||
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pcDNA-f:PGC1 Resource Report Resource Website |
RRID:Addgene_1026 | PPAR gamma coactivator 1 alpha | Mus musculus | Ampicillin | PMID:10983978 | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:16 | 0 | ||
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p11d-tRPA(123) Resource Report Resource Website 1+ mentions |
RRID:Addgene_102613 | RPA1 | Homo sapiens | Ampicillin | PMID:8157639 | Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 1 | ||
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p11d-sctRPA Resource Report Resource Website |
RRID:Addgene_102614 | RPA1 | Saccharomyces cerevisiae | Ampicillin | PMID:9730822 | RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed) | Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |
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pMA_Level 1A Resource Report Resource Website |
RRID:Addgene_102701 | J23106:B0034:spisPink:rrnBT1-T7TE | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:18 | 0 | |||
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pMA_Level 1Γ Resource Report Resource Website |
RRID:Addgene_102703 | J23106:B0034:spisPink:rrnBT1-T7TE | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:18 | 0 | |||
|
APPL1-N308D-mCherry Resource Report Resource Website |
RRID:Addgene_102784 | APPL1 | Homo sapiens | Kanamycin | PMID: | *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function. | Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | changed asparagine 308 to aspartate; *(see below) | 2022-04-22 03:15:19 | 0 |
|
Rab5-L38R-mCherry Resource Report Resource Website |
RRID:Addgene_102785 | Rab5a | Homo sapiens | Kanamycin | PMID: | mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame. | Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Leucine 38 changed to Arginine | 2022-04-22 03:15:19 | 0 |
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pET_RTX_(exo-) Resource Report Resource Website |
RRID:Addgene_102786 | reverse transcription xenopolymerase with 3'-5' deficient exonuclease | Other | Ampicillin | PMID:27339990 | Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:19 | 0 | ||
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hisSUMO-ZFP57 Resource Report Resource Website |
RRID:Addgene_102716 | Zinc finger protein 57 | Homo sapiens | Ampicillin | PMID:29159284 | Backbone Marker:NEB; Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:18 | 0 | ||
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pMA_Auxiliary 4Γ Resource Report Resource Website |
RRID:Addgene_102714 | Linker sequence | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:18 | 0 | |||
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pMA_Auxiliary 2 Resource Report Resource Website |
RRID:Addgene_102710 | Linker sequence | Kanamycin | PMID:29293531 | Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:18 | 0 | |||
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Lv EF1a HP1cs-Frb1x PGK Puro Resource Report Resource Website |
RRID:Addgene_102808 | HP1cs-Frb1x | Ampicillin | PMID:28916764 | Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:20 | 0 | |||
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Lv EF1a HP1cs-Frb2x PGK Puro Resource Report Resource Website |
RRID:Addgene_102809 | HP1cs-Frb2x | Ampicillin | PMID:28916764 | Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:20 | 0 | |||
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pTRE-T2-miR-PURO Resource Report Resource Website |
RRID:Addgene_102646 | Ampicillin | PMID: | In fusion cloning was used to insert a synthetic DNA template containing the mIR cassette from the pCDNA 6.2-GW/miR into the EcoRI and XbaI site of pTRE-T2-Tight. I engineered two BsaI sites into the miR cassette so users can digest the vector with BsaI for directional cloning of Blockit microRNAs using the Block-IT RNAi designer engine from Invitrogen. To make the BsaI sites unique I mutated out Bsa I sites in the original pTRE-T2 Tight vector and also removed XhoI and XbaI sites by site-directed mutagenesis from the pTRE-T2 vector and the corresponding puromycin/hygromycin resistance genes. I also dropped in a unique NheI and EagI restriction site between the ampicillin gene and the pMB101 origin of replication sequence so that the puromycin and hygromin genes could be placed into the pTRE-T2-miR vector with the infusion cloning method. Investigators can remove the miR cassette from the pTRE-T2 puromycin/hygromycin vector and using cloning methods to insert any cDNA of interest into the site for doxycline-inducible expression of the target cDNA. A single vector can be utilized from the production of tetracycline inducible miRNAs or cDNAs. For puromycin use .25 micrograms/ml to .50 micrograms/ml to get clones after a couple of weeks. Growth response curve tested in 293HEK cells. Suggest methylation deficient cells from NEB to alleviate methylation sensitive restriction sites like Xba I. However not recommended for long-term propagation and storage. | Backbone Marker:Markus Ralser (Addgene plasmid # 19407), Clontech = pTRE-T2; Vector Backbone:pTRE-Tight2; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |||
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pcDNA3-neo-cterminal-3HA Resource Report Resource Website |
RRID:Addgene_102643 | Ampicillin | PMID: | No insert, but contains 3 HA epitopes for c-terminal tagged proteins | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3_neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |||
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pcDNA3-neo-Strep_Flag_Nterm Resource Report Resource Website |
RRID:Addgene_102644 | Ampicillin | PMID:27365400 | No insert, but contains a single N-terminus Strepavidin binding peptide and Flag epitopes | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3_neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |||
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pAAV-CamKIIa-C1V1::FusionRed::Kv2.1 Resource Report Resource Website |
RRID:Addgene_102771 | C1V1::FusionRed::Kv2.1 | Other | Ampicillin | PMID: | Backbone Size:5366; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:19 | 0 | ||
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S449F EPHA3 pcDNA3.1 Resource Report Resource Website |
RRID:Addgene_102748 | EPHA3 | Homo sapiens | Ampicillin | PMID:22829656 | Please note last 10 amino acids in C-terminal are omitted in these constructs. These series of EPHA3 constructs carry missense mutations found in lung and colon cancer. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+)/myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S449F (TCC > TTC) | 2022-04-22 03:15:19 | 0 |
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