Species: Homo sapiens
Genetic Insert: hTRIM28
Vector Backbone Description: Backbone Marker:Barton Lab; Vector Backbone:pCMX-(Flag)x2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124960 Copy
Species: Homo sapiens
Genetic Insert: human LDLR
Vector Backbone Description: Backbone Marker:BCM; Backbone Size:24000; Vector Backbone:pD21; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: As this plasmid is very large, NGS had difficulty sequencing it. There are several differences between Addgene's NGS and depositors sequence. We recommend running additional diagnostic tests before use.
Depositor also recommends growing plasmid at 30 degrees C due to their size.
Proper citation: RRID:Addgene_124964 Copy
Species: Homo sapiens
Genetic Insert: hTRIM28
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: TRIM28 contains P719L mutation compared with NM_005762.3. No effect on plasmid function.
Proper citation: RRID:Addgene_124955 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124926 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124927 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124928 Copy
Species: Homo sapiens
Genetic Insert: CRE-M AR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12497 Copy
Species: Homo sapiens
Genetic Insert: CRE-M AR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: To generate a fusion
gene consisting of cre recombinase and the AR LBD, plasmid
p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into
pCMV-CRE-M(BglII) by digesting two separate aliquots of
pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The
ligation of these three fragments resulted in the generation of
pCMV-CRE-M-AR(wt).
Proper citation: RRID:Addgene_12494 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124923 Copy
Species: Homo sapiens
Genetic Insert: TSC2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Compared to GenBank reference sequence NP_001107854.1, the TSC2 insert contains a S802R mutation.
Proper citation: RRID:Addgene_12132 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM4D(Gi)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121538 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121492 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121493 Copy
Species: Homo sapiens
Genetic Insert: Human PDX1 (open)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: PDX1 PCR'd from HsCD00082591 (DNASU)
Proper citation: RRID:Addgene_121745 Copy
Species: Homo sapiens
Genetic Insert: Human PDX1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: PDX1 PCR'd from HsCD00082591 (DNASU)
Proper citation: RRID:Addgene_121744 Copy
Species: Homo sapiens
Genetic Insert: cdc42
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12175 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GACh2.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121921 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GRAB-ACh3.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121923 Copy
Species: Homo sapiens
Genetic Insert: Mst1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3M; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12204 Copy
Species: Homo sapiens
Genetic Insert: Mst1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3H; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12203 Copy
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