Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson lab; Backbone Size:4980; Vector Backbone:pAAV-EF1α1.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore region). Multiple digestions were done to verify the vector structure. The construct was tested in vitro.
Proper citation: RRID:Addgene_122101 Copy
Species: Synthetic
Genetic Insert: β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125236 Copy
Species: Synthetic
Genetic Insert: mCherry-GS linker-Zdk1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125226 Copy
Species: Synthetic
Genetic Insert: gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125225 Copy
Species: Synthetic
Genetic Insert: NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125228 Copy
Species: Synthetic
Genetic Insert: Lyn-Phospho-Activity-Reporter, Empty
Vector Backbone Description: Backbone Marker:Addgene plasmid # 60523; Backbone Size:5661; Vector Backbone:pSBbi-Pur; Vector Types:Mammalian Expression, Other, Transposon; Bacterial Resistance:Ampicillin
References:
Comments: Targeted to membrane by lipid raft-targeting peptide (GCIKSKRKD) derived from human kinase LYN.
Proper citation: RRID:Addgene_125205 Copy
Species: Synthetic
Genetic Insert: cpVenus[E172]
Vector Backbone Description: Backbone Marker:Addgene plasmid # 60523; Backbone Size:5661; Vector Backbone:pSBbi-Pur; Vector Types:Mammalian Expression, Other, Transposon; Bacterial Resistance:Ampicillin
References:
Comments: Not membrane-targeted
Proper citation: RRID:Addgene_125202 Copy
Species: Synthetic
Genetic Insert: YdLight1
Vector Backbone Description: Backbone Size:3983; Vector Backbone:pEGFP_N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_125705 Copy
Species: Synthetic
Genetic Insert: ymNeongreen-T2A-mCherry
Vector Backbone Description: Vector Backbone:pDRF1-GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125700 Copy
Species: Synthetic
Genetic Insert: SYK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego; Backbone Size:5505; Vector Backbone:pcDNA3.1’; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125729 Copy
Species: Synthetic
Genetic Insert: nScCas9-ABE7.10, wheat/human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125820 Copy
Species: Synthetic
Genetic Insert: A3A-PBE, wheat codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125818 Copy
Species: Synthetic
Genetic Insert: nSpCas9-ABE7.10 base changer, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125810 Copy
Species: Synthetic
Genetic Insert: FnCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125811 Copy
Species: Synthetic
Genetic Insert: LbCas12a, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125812 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG, human codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125809 Copy
Species: Synthetic
Genetic Insert: Endlinker
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_125761 Copy
Species: Synthetic
Genetic Insert: Endlinker
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_125762 Copy
Species: Synthetic
Genetic Insert: Endlinker
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pUC57-Kan-mini; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_125764 Copy
Species: Synthetic
Genetic Insert: LbCas12a, rice codon optimized
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_125803 Copy
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