Species: Synthetic
Genetic Insert: hU6::SaCas9 gRNA scaffold + RIP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMAGIC Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. SaCas9 gRNA scaffold derived from PMID: 26721686
Proper citation: RRID:Addgene_121815 Copy
Species: Synthetic
Genetic Insert: mU6::xCas9(3.7) gRNA scaffold
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pDONR221 P1-P5r; Vector Types:Synthetic Biology, Other, pMAGIC Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. x/SpCas9 gRNA scaffold derived from PMID: 26671237
Proper citation: RRID:Addgene_121816 Copy
Species: Synthetic
Genetic Insert: mCherry + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121773 Copy
Species: Synthetic
Genetic Insert: HA epitope tag-polyA + CMV::TETa-polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TETa sequence derived from Tet-On Advanced system (Clontech)
Proper citation: RRID:Addgene_121802 Copy
Species: Synthetic
Genetic Insert: polyA + EF1a::TETa-polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TETa sequence derived from Tet-On Advanced system (Clontech)
Proper citation: RRID:Addgene_121805 Copy
Species: Synthetic
Genetic Insert: HA epitope tag-polyA + EF1a::TETa-polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TETa sequence derived from Tet-On Advanced system (Clontech)
Proper citation: RRID:Addgene_121806 Copy
Species: Synthetic
Genetic Insert: polyA + RIP::TETa-polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: TETa sequence derived from Tet-On Advanced system (Clontech)
Proper citation: RRID:Addgene_121809 Copy
Species: Synthetic
Genetic Insert: myc epitope tag + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121760 Copy
Species: Synthetic
Genetic Insert: HA epitope tag + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121756 Copy
Species: Synthetic
Genetic Insert: 3x HA epitope tag + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121757 Copy
Species: Synthetic
Genetic Insert: FLAG epitope tag + WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121758 Copy
Species: Synthetic
Genetic Insert: OLLAS epitope tag + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121752 Copy
Species: Synthetic
Genetic Insert: 6x His epitope tag + polyA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121754 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4657; Vector Backbone:JN607; Vector Types:Mammalian Expression, Other, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Sleeping Beauty ITR sequences derived from Addgene #26563
Proper citation: RRID:Addgene_121866 Copy
Species: Synthetic
Genetic Insert: BirA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121907 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4575; Vector Backbone:JN607; Vector Types:Mammalian Expression, Other, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Sleeping Beauty ITR sequences derived from Addgene #26559
Proper citation: RRID:Addgene_121862 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4261; Vector Backbone:JN607; Vector Types:Mammalian Expression, Other, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Sleeping Beauty ITR sequences derived from Addgene #26561
Proper citation: RRID:Addgene_121864 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4462; Vector Backbone:JN607; Vector Types:Mammalian Expression, Other, Sleeping Beauty Transposon, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Sleeping Beauty ITR sequences derived from Addgene #26562
Proper citation: RRID:Addgene_121865 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:3806; Vector Backbone:JK104; Vector Types:Mammalian Expression, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121855 Copy
Species: Synthetic
Genetic Insert: Gateway selection cassette (ccdb gene + Chlor resistance gene)
Vector Backbone Description: Backbone Marker:Newgard Lab; Backbone Size:4007; Vector Backbone:JK104; Vector Types:Mammalian Expression, Other, pMVP Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121856 Copy
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