Species: Mus musculus
Genetic Insert: Stat-1
Vector Backbone Description: Backbone Size:8858; Vector Backbone:pDR111; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_188400 Copy
Species: Mus musculus
Genetic Insert: mouse NFL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4242; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.
Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635).
Proper citation: RRID:Addgene_83127 Copy
Species: Mus musculus
Genetic Insert: IkB alpha M
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
Proper citation: RRID:Addgene_12329 Copy
Species: Mus musculus
Genetic Insert: IkB alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12331 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38012 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182682 Copy
Species: Mus musculus
Genetic Insert: NEMO shRNA1
Vector Backbone Description: Backbone Marker:Available at Addgene (#12084); Backbone Size:7700; Vector Backbone:pSicoR PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Mouse NEMO shRNA1 in pSicoReverse (Puromycin)
forward oligo:
TGGACATGCTGGGTGAAGAATTCAAGAGATTCTTCACCCAGCATGTCCTTTTTTC
reverse oligo :
TCGAGAAAAAAGGACATGCTGGGTGAAGAATCTCTTGAATTCTTCACCCAGCATGTCCA
Proper citation: RRID:Addgene_22505 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38010 Copy
Species: Mus musculus
Genetic Insert: Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182680 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182686 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182685 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.
Proper citation: RRID:Addgene_38009 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_182661 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K468TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_182663 Copy
Species: Mus musculus
Genetic Insert: IkB alpha
Vector Backbone Description: Backbone Size:11500; Vector Backbone:RCAS; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12406 Copy
Species: Mus musculus
Genetic Insert: IkappaBalpga
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44738 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_182656 Copy
Species: Mus musculus
Genetic Insert: wild type mouse neurofilament light chain
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_182659 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K468TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_182658 Copy
Species: Mus musculus
Genetic Insert: Clathrin Light Chain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned from IMAGE clone: 3154277 (MGC:68133).
Proper citation: RRID:Addgene_27680 Copy
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