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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCDF1-mAtf4-HT-puro
 
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RRID:Addgene_101795 Activating transcription factor 4 Mus musculus Ampicillin PMID: Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2022-04-22 03:15:10 0
pGL3-mFgf21pro1-luc
 
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RRID:Addgene_101797 Fibroblast growth factor 21 promoter Mus musculus Ampicillin PMID:26487695 Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2022-04-22 03:15:10 0
pCSN061
 
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RRID:Addgene_101725 S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. Ampicillin PMID:28886218 Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:15:09 0
pAAV hSyn1 CaRhAC T2A tDimer
 
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RRID:Addgene_101722 CatRhAC Other Ampicillin PMID:29799525 Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin E497K,C569D 2022-04-22 03:15:08 0
pAAV hSyn1 CaRhGC wt T2A tDimer
 
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RRID:Addgene_101720 CatRhGC Other Ampicillin PMID:29799525 Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2022-04-22 03:15:08 0
pCRRNA-G20-R18
 
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RRID:Addgene_101817 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
pCRRNA-G0-R20
 
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RRID:Addgene_101818 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
pCRRNA-G11-R18
 
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RRID:Addgene_101815 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
pCRRNA-G14-R18
 
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RRID:Addgene_101816 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
pCRRNA-G14-R11
 
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RRID:Addgene_101811 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
H14-MBP-SUMO-Cys-Linker-BAF_G47E
 
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RRID:Addgene_101779 BAF (BANF1) Homo sapiens Kanamycin PMID:28841419 Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin G47E mutation 2022-04-22 03:15:09 0
YORWΔ22α1
 
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RRID:Addgene_101708 Kanamycin PMID:29132331 Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2α1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:15:08 0
YPRCΔ15Ω2
 
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RRID:Addgene_101709 Spectinomycin PMID:29132331 Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2022-04-22 03:15:08 0
YORWΔ22Ω2
 
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RRID:Addgene_101705 Spectinomycin PMID:29132331 Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2022-04-22 03:15:08 0
YORWΔ22Ω1
 
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RRID:Addgene_101706 Spectinomycin PMID:29132331 Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin 2022-04-22 03:15:08 0
AICSDP-22:CETN2-mTagRFP-T
 
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RRID:Addgene_101781 CETN2 Homology Arms with mTagRFPT-linker Homo sapiens Ampicillin PMID: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2022-04-22 03:15:09 0
pCRRNA-G14-R20
 
Resource Report
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RRID:Addgene_101821 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0
AICSDP-28:TUBA1B-mTagRFP-T
 
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RRID:Addgene_101785 TUBA1B Homology Arms with mTagRFPT-linker Homo sapiens Ampicillin PMID: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2022-04-22 03:15:10 0
pRN1120
 
Resource Report
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RRID:Addgene_101750 Ampicillin PMID:28886218 Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:09 0
pCRRNA-G10-R0
 
Resource Report
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RRID:Addgene_101804 crRNAs targeting GFP and mCherry Synthetic Kanamycin PMID:29519933 Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-04-22 03:15:10 0

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