| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pCDF1-mAtf4-HT-puro Resource Report Resource Website |
RRID:Addgene_101795 | Activating transcription factor 4 | Mus musculus | Ampicillin | PMID: | Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:10 | 0 | ||
|
pGL3-mFgf21pro1-luc Resource Report Resource Website |
RRID:Addgene_101797 | Fibroblast growth factor 21 promoter | Mus musculus | Ampicillin | PMID:26487695 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:10 | 0 | ||
|
pCSN061 Resource Report Resource Website |
RRID:Addgene_101725 | S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. | Ampicillin | PMID:28886218 | Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 0 | |||
|
pAAV hSyn1 CaRhAC T2A tDimer Resource Report Resource Website |
RRID:Addgene_101722 | CatRhAC | Other | Ampicillin | PMID:29799525 | Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | E497K,C569D | 2022-04-22 03:15:08 | 0 | |
|
pAAV hSyn1 CaRhGC wt T2A tDimer Resource Report Resource Website |
RRID:Addgene_101720 | CatRhGC | Other | Ampicillin | PMID:29799525 | Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:08 | 0 | ||
|
pCRRNA-G20-R18 Resource Report Resource Website |
RRID:Addgene_101817 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
pCRRNA-G0-R20 Resource Report Resource Website |
RRID:Addgene_101818 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
pCRRNA-G11-R18 Resource Report Resource Website |
RRID:Addgene_101815 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
pCRRNA-G14-R18 Resource Report Resource Website |
RRID:Addgene_101816 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
pCRRNA-G14-R11 Resource Report Resource Website |
RRID:Addgene_101811 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
H14-MBP-SUMO-Cys-Linker-BAF_G47E Resource Report Resource Website |
RRID:Addgene_101779 | BAF (BANF1) | Homo sapiens | Kanamycin | PMID:28841419 | Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G47E mutation | 2022-04-22 03:15:09 | 0 | |
|
YORWΔ22α1 Resource Report Resource Website |
RRID:Addgene_101708 | Kanamycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2α1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:08 | 0 | ||||
|
YPRCΔ15Ω2 Resource Report Resource Website |
RRID:Addgene_101709 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:15:08 | 0 | ||||
|
YORWΔ22Ω2 Resource Report Resource Website |
RRID:Addgene_101705 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:15:08 | 0 | ||||
|
YORWΔ22Ω1 Resource Report Resource Website |
RRID:Addgene_101706 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:15:08 | 0 | ||||
|
AICSDP-22:CETN2-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101781 | CETN2 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:09 | 0 |
|
pCRRNA-G14-R20 Resource Report Resource Website |
RRID:Addgene_101821 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 | ||
|
AICSDP-28:TUBA1B-mTagRFP-T Resource Report Resource Website |
RRID:Addgene_101785 | TUBA1B Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | PMID: | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2022-04-22 03:15:10 | 0 |
|
pRN1120 Resource Report Resource Website |
RRID:Addgene_101750 | Ampicillin | PMID:28886218 | Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:09 | 0 | ||||
|
pCRRNA-G10-R0 Resource Report Resource Website |
RRID:Addgene_101804 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:10 | 0 |
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