Searching the RRID Resource Information Network
Species: Danio rerio
Genetic Insert: itga9 sgRNA7 target sequence
Vector Backbone Description: Backbone Size:2126; Vector Backbone:pDR274; Vector Types:CRISPR, Other, sgRNA generation vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_132981 Copy
Species: Danio rerio
Genetic Insert: itga9 sgRNA4 target sequence
Vector Backbone Description: Backbone Size:2126; Vector Backbone:pDR274; Vector Types:CRISPR, Other, sgRNA generation vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_132980 Copy
Species: Danio rerio
Genetic Insert: gata2a
Vector Backbone Description: Backbone Size:2543; Vector Backbone:pDONR221; Vector Types:Other, Gateway vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_132977 Copy
Species: Danio rerio
Genetic Insert: foxc1a
Vector Backbone Description: Backbone Size:2543; Vector Backbone:pDONR221; Vector Types:Other, Gateway vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_132971 Copy
Species: Danio rerio
Genetic Insert: gata2a endothelial enhancer (x6) with a b-actin basal promoter
Vector Backbone Description: Backbone Size:2643; Vector Backbone:pDONR221; Vector Types:Other, Gateway vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_132968 Copy
Species: Danio rerio
Genetic Insert: gcsf
Vector Backbone Description: Backbone Size:4094; Vector Backbone:pCS2+; Vector Types:Other, mRNA synthesis; Bacterial Resistance:Ampicillin
References:
Comments: The gcsf clone was cloned into a modified pCS2+ vector (pCS2+ TA; PMID: 24918328), a kind gift of Dr. Robert Bryson-Richardson (Monash University).
Proper citation: RRID:Addgene_132947 Copy
Species: Danio rerio
Genetic Insert: plcg1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS.
For riboprobe, digest with MluI and use SP6 polymerase.
Proper citation: RRID:Addgene_13058 Copy
Species: Danio rerio
Genetic Insert: tie2
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobe, digest with Eco 01091 and use T3 polymerase. The depositor noted that the tie2 Q817R mutation found in Addgene's quality control sequence does NOT affect plasmid function.
Proper citation: RRID:Addgene_13064 Copy
Species: Danio rerio
Genetic Insert: flt4
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobe, digest with EcoRI and use T7 polymerase.
Proper citation: RRID:Addgene_13062 Copy
Species: Danio rerio
Genetic Insert: ephrin B2
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobe, digest with SalI and use T7 polymerase.
Proper citation: RRID:Addgene_13063 Copy
Species: Danio rerio
Genetic Insert: Homologous arm
Vector Backbone Description: Backbone Size:7326; Vector Backbone:pTol2-eab2-GG; Vector Types:Other, Zebrafish Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182158 Copy
Species: Danio rerio
Genetic Insert: Homologous arm
Vector Backbone Description: Backbone Size:7055; Vector Backbone:pTol2-eab2-AG; Vector Types:Other, Zebrafish Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182159 Copy
Species: Danio rerio
Genetic Insert: Homologous arm
Vector Backbone Description: Backbone Size:7588; Vector Backbone:pTol2-eab2-GA; Vector Types:Other, Zebrafish Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_182160 Copy
Species: Danio rerio
Genetic Insert: cytosolic phospholipase a2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163844 Copy
Species: Danio rerio
Genetic Insert: BMP2b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Probe: cut with EcoRI, transcribe with T3 RNA polymerase.
Proper citation: RRID:Addgene_16915 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117766 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117768 Copy
Species: Danio rerio
Genetic Insert: TagRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2329; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development
Proper citation: RRID:Addgene_117801 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117792 Copy
Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at
Proper citation: RRID:Addgene_117793 Copy
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