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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 4 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
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Notes:
Proper citation: RRID:TSC_SD01065 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 [B or C3] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
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Notes:
Proper citation: RRID:TSC_SD00371 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MPR1; ADA2/ada2-1[Δ1-537(::neo4, KanR2)]; pm-r, VII)
Affected Genes: ADA2 (TTHERM_00790730)
Genomic Alteration: Micronucleus: PAR1 gene partially replaced by neo4-KanR2 cassette. Macronucleus: ADA2 gene partially replaced by neo4-KanR2 cassette.
Availability:
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Notes: The knockout also deletes 213bp of the 5' flanking region and introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01741 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
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Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01864 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (rnh1.3[HA]; pm-r, VII)
Affected Genes: RNH13 (TTHERM_00283900)
Genomic Alteration: Macronucleus: RNH1.3 with HA tag
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Notes: All Somatic RNH1.3 was replaced by RNH1.3 HA construct. Not sure where the neo3 is. CU428 background.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02710 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1R (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
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Notes: Large deletion. May be missing entire right arm of chromosome 1.
Proper citation: RRID:TSC_SD00777 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VI)
Affected Genes:
Genomic Alteration:
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Notes: B strain star line. Lacks a genetically functional micronucleus.
Proper citation: RRID:TSC_SD00022 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E433D,E435D]; pm-r, ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU1 has only (E431, 433, 435D)) no WT
BTU2 is KO
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,433,435D).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01914 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, II)
Affected Genes:
Genomic Alteration:
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Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00032 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rmm12[C3]/rmm12[C3] (mp-s, pm-s, rrm12, III)
Affected Genes:
Genomic Alteration:
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Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01641 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 1R [chx1-1/chx1-1[B] or CHX1/CHX1[C3]] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
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Proper citation: RRID:TSC_SD00397 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K130R]; pm-r)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutation K130R
Availability:
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Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated K130R. no ubiquitination of H2AF/Z. Can mate with T42E1
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02458 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1 (btu1[∆, H4-neo]; pm-r, IV)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: histone H4 promoter sequence then neo coding to replace the BTU2 coding region.
Availability:
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Notes: Complete disruption of BTU2 by an H4-neo cassette. CU428xB2086 electroporation transformation with histone H4 promoter sequence then neo coding to replace the BTU2 coding region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01922 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
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Notes:
Proper citation: RRID:TSC_SD01010 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type D (wild type D; wild type D, I)
Affected Genes:
Genomic Alteration:
Availability:
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Synonyms:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01495 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E441A, E443A, E445A,E446A,E447A ]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E441A, E443A, E445A, E446A, E447A or neo replacing coding of some ATU1 genes
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Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU5A derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E441A, E443A, E445A,E446A,E447A. Slow growth, slow swimming, cytokinesis arrest.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02226 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Del 3L (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
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Notes: Deletion on left arm of chromosome 3.
Proper citation: RRID:TSC_SD00969 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: RDR1/rdr1[∆::neo2] (cy-r, ?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Micronucleus: neo2 into RDR1
Availability:
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Notes: Cross RDP1del XCU427. No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02508 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, II)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Availability:
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Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02470 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcl1[∆?::neo3]/dcl1[∆?::neo3] (Complete KO of DCL1 with neo3, ?)
Affected Genes: DCL1 (TTHERM_00284230), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: HA tagged TWI 11 Macronucleus: Neo3 KO of DCL1
Availability:
References:
Synonyms:
Notes: Complete somatic DCL1 knockout with HA-tagged Twi11 in the Mic, to study Twi11 localization and loading with scnRNA.
Mates with ∆DCL1(s)HAT11(5-1).
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02591 Copy
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