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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
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Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01250 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?HHT3/hht3[3’neo2, HAn]; pm-r, II)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
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Notes: HA-tagged hv2 mac transformant. Checked by western blot. Hahv2 is expressed. This strain can be used to check HA-hv3 localization in conjugated cells by mating with HA-hv2/CU428.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01892 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht4[∆::neo2]/hht4[∆::neo2]
Affected Genes: HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT4 is replaced by neo2
Availability:
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Notes: Germline HHT4 KO heterokaryon HHT4 coding is replaced by neo2, * side
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01377 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, II)
Affected Genes:
Genomic Alteration:
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Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01651 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2,∆,HAHAn(::TCD4)]; pm-r, VII)
Affected Genes: HPL4 (TTHERM_00585180)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD4, neo2 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Availability:
References:
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Notes: HA2-TCD4 overexpession from MTT1 locus, neo 2 upstream. HA-Tcd3p localizes to new mac. TTHERM_00585190
Adjacent to TCD3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02500 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1; tyr-9/tyr-9 (gal1-1; tyr-9; gal-r; tyr auxotroph, II)
Affected Genes:
Genomic Alteration:
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Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01535 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/CHX1; PMR1/pmr1-1; GAL1/gal1-1; CNJ1/cnj1-1 (?; ?, II)
Affected Genes:
Genomic Alteration:
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Notes: Macronucleus phenotype and genotype are undetermined.
Proper citation: RRID:TSC_SD00203 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K33Q,K38Q,K40Q,K50Q,K51Q)]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to alanine (charge patch) and 5 other sites to glutamine.
Availability:
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Notes: Complete somatic tranformant of CU428 cells with pQ5A5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines, neo 2 cassette in 3’ flanking region for selection. See paper.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02504 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2] (hta1[∆::neo2], htax[∆::neo2]/htax [S1P,,K5R,K8R,K10R, K12R,K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: HTAX and HTA1 KO with neo Macronucleus: Rescue with mutated HTAX with S1P, , K5R, K8R, K10R, K12R, K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A
Availability:
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Notes: HTAX S1P+5R+4A+3R mutation somatic rescue strain of HTA1, HTA2 double knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02198 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF2; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF2
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Synonyms:
Notes: EF-hand mutant allele rescue of cen1∆ - D72A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03285 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration:
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Notes: CET of CU428 x B2086 with plamid XL202, HTA2 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02877 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
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Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-r, mat2. Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01268 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (mp-r?, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ
Availability:
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Notes: HTA/HTZ1 triple KO heterokaryons;From mating of h∆∆HTA-G6A and h∆HTZ1-G311A1; made with B*VII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02633 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,∆,::(HHT2,GFPc)]/hht3[5’neo2,∆,(::HHT2,GFPc)] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 into HHT3 locus
Availability:
References:
Synonyms:
Notes: non-star side of H3.2+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01946 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/HTB1 ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: HTB1
Availability:
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Notes: ∆∆HTB knockout heterokaryons rescued by HTB1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02088 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX1/CHX1; mpr1?/mpr1? (mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
Availability:
References:
Synonyms:
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1 G1 14B,
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01395 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml) Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL5-1 and 5-2.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02246 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4R] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4R
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4R.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02093 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-4/hpo1-4 (hpo1-4, IV)
Affected Genes: HPO1 (hypoangular)
Genomic Alteration:
Availability:
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Notes: A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. Similar to hpo1-1, though much less extensively studied. A sister clone to IA480.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01443 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1, and for HHT2 have mutations S10A and S28A
Availability:
References:
Synonyms:
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #6-later after seq.transformant found a wrong nucleotide~25 bases upsteam of ATG, transformant seems normal.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02376 Copy
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