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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 [B or C3] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00372 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Availability:
References:
Synonyms:
Notes: Don’t know if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02312 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2 (hpo1-2, VI)
Affected Genes: HPO1 (hypoangular)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. The phenotype is more strongly expressed at 28° C and less strongly at 39° than it is in hpo1-1 (IA393).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01465 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560 + TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Availability:
References:
Synonyms:
Notes: homozygous, Neo cassette knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01860 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmA-3/psmA-3 (psmA-3, II)
Affected Genes: PSMA (pseudomacrostome A)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia.(39°). Same phenotype as psmA-1 IV (IA223) but different conditions of expression: Detailed phenotypic analysis was carried out on the sister clone, IA486.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01467 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-1/cdaC-1; cdaE-2/cdaE-2 (cdaC-1; cdaE-2; cdaC, cdaE, IV)
Affected Genes: CDAC (cell division arrest C) , CDAE (cell division arrest E)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Joint expression of cdaC and cdaE patterns of fission arrest.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01587 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: somatic, H1 wt-GFP tagged; pushed to completion (hho1[3’neo2,GFPc]; pm-r, GFP positive, VII)
Affected Genes: HHO1, Histone H1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: GFP tagged HHO1
Availability:
References:
Synonyms:
Notes: This is the somatic replacement strain GFP tagged wt H1 replaced the endogenous H1 flanking neo2 cassette was used as resistance marker
From the Gorovsky Lab, University of Rochester.
Proper citation: RRID:TSC_SD01744 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htay[∆123-388::neo3]/htay[∆123-388::neo3] (HTAY/HTAY, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Micronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene
Availability:
References:
Synonyms:
Notes: Knockout Heterokaryon of HTAY gene. Different mating type from 6-2, 6-3, 6-6, 6-12. Same as 3-2-5
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02680 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpH-1/mpH-1 (mpH-1, IV)
Affected Genes: MPH (membranellar pattern H)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Non-conditional formation of two instead of three membranelles in the oral apparatus. Similar phenotype to mpG (IA352), but with lower penetrance of 2-membranellar phenotype.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01235 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: psmC-1/psmC-1 (psmC-1, II)
Affected Genes: PSMC (pseudomacrostome C)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Elongated phenotype with development of large oral-replacement or misplaced cell division oral primordia, especially in rich medium, at 39o C. The only known mutant allele of this gene. Located on chromosome 5. Expression is highly medium-sensitive as well as temperature-sensitive.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01514 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (dyh7::neo3; pm-r, ?)
Affected Genes: DYH7 (TTHERM_00912290)
Genomic Alteration: Macronucleus: dyh7::neo3
Availability:
References:
Synonyms:
Notes: Submitted by Todd Hennessey, University at Buffalo
Proper citation: RRID:TSC_SD00704 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A (neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Availability:
References:
Synonyms:
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01887 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01375 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TTHERM_01084190-1[∆::NEO2] (WT; WT, ?)
Affected Genes: TTHERM_01084190 (BBS1)
Genomic Alteration: Macronucleus: none
Availability:
References:
Synonyms:
Notes: Mates with UCB1002
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03310 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01653 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MTT1/MTT1 (MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; Mtt1p/Cna1p-GFPc, II)
Affected Genes: CenpA
Genomic Alteration: Macronucleus: GFP cterminal tagged CNA1 coding, inserted into MTT1 locus
Availability:
References:
Synonyms:
Notes: MTT-CenpA-GFP was transformed into B2086 at MTT1 locus somatially. Not completed - grow in 0.5 ug/mlCd++and 200ug/ml paromomycin.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01777 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Availability:
References:
Synonyms:
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Non-star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01932 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP,∆HHT3,∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Availability:
References:
Synonyms:
Notes: H3.2-GFP homozygous heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01778 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous KO with neo cassette ∆HMGB/∆HMGB (pm-r, mp-?
)
Affected Genes: HMGB (TTHERM_00660180)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Obtained thru mating two homozygous germline KO strains. Complete replacement in both mic and mac. Same as -B2, independent strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01384 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Availability:
References:
Synonyms:
Notes: somatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02234 Copy
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