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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Micronucleus: H4-neo replaces coding of BTU1, heterozygous
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTUI knockout. Derived from Cu428 X B2086 transformed w/ BHAB-1(construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to BIKO-2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02753 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]; MTT1/mtt1-?[∆(::BTU1)HAc]; pm-r, ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: HA c-terminal tagged BTU1 into MTT1 locus
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, transformed by BTU-HA TTMet construct. The transformants are dependent on Cadmium, 0.25ug/ml.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02236 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, ?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Macronucleus: HA-FLAG tag of RDR1
Availability:
References:
Synonyms:
Notes: Shot plasmid pRDP1::TAG-neo3 #110 into conjugating CU428XB2086 but only got somatic strains, slow growth.
No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02512 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]::SFR13[5'FLAG::BSR]::SFR13 3'; WT cell size and number of basal bodies., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2] Macronucleus: FLAG-SFR13 rescue in two parts (see comments)
Availability:
References:
Synonyms:
Notes: sfr13 complete null rescue. First a 5' part of the gene was introduced into the sfr13 locus with BSR selection that included a 5'FLAG tag. Second, the remainder of the gene was introduced to the KO locus with the 5' BSR insertion, and rescue of the small cell size phenotype was selected for.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03298 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ngoA[∆::neo3]/ngoA[∆::neo3] (ngoA[∆::neo3]
; pm-r, NgoA KO, ?)
Affected Genes: NgoA (TTHERM_00655890)
Genomic Alteration: Micronucleus: neo3 into NgoA coding Macronucleus: neo3 into NgoA coding
Availability:
References:
Synonyms:
Notes: It’s a homozygous clone of KO of NgoA gene in both mac and mic grew up from sinle cell. the KO construct is: Neo gene driven by MTT promoter(2.5kb), and this is in opposite direction in NgoA flanking. NgoA KO-12 can mate with NgoA KO-7
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02488 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3]
(cnjB[∆?::neo3], ?)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: Neo3 replaces part of coding Macronucleus: Neo3 replaces part of coding
Availability:
References:
Synonyms:
Notes: Homozygote CnjB KO Homokaryon. Partial cnjB coding sequences were replaced by neo3.
This strain was supposed to be pm-r but thawed pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02761 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆?::neo3]/twi1[∆?::neo3] (twi1[∆?::neo3]; eri1[∆?::neo3]; same as ∆Twi1)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: TWI1 ko Macronucleus: twi1 kO and ERI1 KO
Availability:
References:
Synonyms:
Notes: Eri1 KO construct was shot into twi1 KO homozygous homokaryon (Neo3 gene is lost in mac, strain is pm-s).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02007 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 8/26/2008 from the pond designated SG69-1 in Guys Mills, PA (lat. 41 37.174, long. -79 55.095, elevation 1549 ft, nearest road is Carpenter Road).
Geographical area: Western Pennsylvania
Collection number: 893
Has a micronucleus.
Cech's self-splicing intron is present. Cytochrome oxidase I haplotype = ANF1
Proper citation: RRID:TSC_SD01556 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01955 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E443D,E445D E446D,E447D]; ATU1 with E443D, E445D,E446D,E447D or neo replacing coding of some ATU1 genes, pm-r, cy-s,mp-?
)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E443D, E445D, E446D, E447D or neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU-4D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E443D, E445D,E446D,E447D
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01837 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL1-3,4,5 and 2-5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02250 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01166 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T146V)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pT146V-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 T146V gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus. Single cell (a)
These are same as previous ones, but new transformants, (one week old) not single celled and haven’t tested genotype yet
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02531 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01165 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vac?/vac? (mpr1, vac?; mp-r, vac-, oral apparatus-, contractil vacuole pore-, rounded, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Complementation group could not be determined because of sterility of the mutant.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01603 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpC-2/mpC-2 (mpC-2, VII)
Affected Genes: MPC (membranellar pattern C)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Formation of a fourth membranelle in the oral apparatus. Located on chromosome 3, probably 3L. Allelic to mpC-1 (IA308), with similar oral expression and no pleiotropic effects on cell division. Penetrance increased at higher temperatures.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01451 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mic still from VB1 cell (btu1[∆2,3,4]/btu1[∆2,3,4]; BTU1 has M--- ( REI removed at start of coding); no longer taxol hypersensitive or oryzalin resistant since back to wt L350)
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Macronucleus: Removed the amino acids REI at positions 2, 3, 4 ibeta tubulin 1 gene
Availability:
References:
Synonyms:
Notes: Somatically transformed VB1 cells with BTU1 p94c construct. p94c changes MREI at begining of coding to remove the REI. Also put the missing “C†back into the BTU1 coding region (~ position + 1040)
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02269 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02148 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2; cdaA-2/cdaA-2 (hpo1-2; cdaA-2; hpo1-2, cdaA-2, III)
Affected Genes: HPO1(hypoangular1), CDAA (cell division arrestA)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This clone combines a temperature-sensitive fission arrest with a hypoangular phenotype. It was used to generate and investigate "left handed" hypoangular cells
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01579 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ K125R]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. mutated HTA1 rescue ∆HTA1∆HTA2 double knockout heterokaryon. No H2A.1 ubiquitination . Can mate with D13D1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02390 Copy
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