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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00054605
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: srh-146(yum2719) srh-147(yum2720) srh-148(yum2721) srh-166(yum2716) srh-167(yum2717) srh-169(yum2718) V
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054605 Copy
http://www.wormbase.org/db/get?name=WBStrain00054566
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: inx-14(ag17); zwIs142[Psra-11::GFP]
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054566 Copy
http://www.wormbase.org/db/get?name=WBStrain00054567
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: acc-2(ok2216); kyEx3801[Psra-11::ChR2::GFP, Punc-122::dsRed]; zwEx277[Pflp-18::loxP::LacZ::STOP::loxP::acc-2(cDNA), Pflp-18::lox-P::LacZ::STOP::loxP::mCherry::SL2::GFP, Pgpa-14::Cre]
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054567 Copy
http://www.wormbase.org/db/get?name=WBStrain00054569
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: zwEx287[Punc-171::: inx-12ss, Punc-171:: inx-12as]; zwIs142[Psra-11::GFP]
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054569 Copy
http://www.wormbase.org/db/get?name=WBStrain00054602
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: srh-195(yum2500) II; srh-185(yum2493) srh-186(yum2494) srh-187(yum2495) srh-190(yum2496) srh-192(yum2497) srh-193(yum2498) srh-194(yum2499) srh-199(yum2501) V
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054602 Copy
http://www.wormbase.org/db/get?name=WBStrain00054607
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: srh-146(yum2719) srh-147(yum2720) srh-148(yum2721) srh-149(yum2722) srh-154(yum2723) srh-159(yum2724) srh-166(yum2716) srh-167(yum2717) srh-169(yum2718) srh-174(yum2729) srh-177(yum2730) srh-178(yum2731) V
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054607 Copy
http://www.wormbase.org/db/get?name=WBStrain00054608
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: srh-146(yum2719) srh-147(yum2720) srh-148(yum2721) srh-149(yum2722) srh-154(yum2723) srh-159(yum2724) srh-166(yum2716) srh-167(yum2717) srh-169(yum2718) srh-174(yum2729) srh-177(yum2730) srh-178(yum2731) srh-179(yum2732) srh-180(yum2733) srh-183(yum2736) V
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054608 Copy
http://www.wormbase.org/db/get?name=WBStrain00054609
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: srh-288(yum2617) srh-289(yum2618) srh-290(yum2619) V
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054609 Copy
http://www.wormbase.org/db/get?name=WBStrain00054551
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: csr-1(fj160); CeRep55 quadruple deletions (fj115, fj85, fj123, and fj120) X
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054551 Copy
http://www.wormbase.org/db/get?name=WBStrain00054553
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: csr-1(fj163fj150) CeRep55 quadruple deletions (fj115, fj85, fj123, and fj120) X
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054553 Copy
http://www.wormbase.org/db/get?name=WBStrain00054550
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001860(him-1)
Genomic Alteration: WBGene00001860(him-1)
Availability: unknown
References:
Synonyms: him-1(e879) I; fjDf1 fjDf2 fjDf3 fjDf4 X.
Notes: The CeRep55_X quadruple-deletion mutant does not exhibit a clear Him phenotype, but the Him phenotype of the him-1(e879) mutant is enhanced by the CeRep55_X quadruple deletions. CeRep55 quadruple deletion: fjDf1 (also known as fj115); fjDf2 (aka fj85); fjDf3 (aka fj123); fjDf4 (aka fj120) X. This strain lacks four major clusters of CeRep55 repeats on the X chromosome. The condensation of unpaired X chromosomes in male testes is insufficient. CeRep55 is a class of minisatellite sequences consisting of a 27-nt tandem repeat that is present on all chromosomes. Some CeRep55 clusters express long non-coding RNAs and small RNAs. Each of the four deletion sites was designed to acquire a sequence tag (TGTACAGGAAACAGCTATGACC; similar to M13 reverse) instead of the CeRep55 tandem repeats. The deletions of CeRep55 clusters can be checked by PCR with the following primers: fjDf1 in Y73B3A, CAACCTGACTCTCGCCAAGAC and GGAGAAGTAGGCGTGTCAGTTA; fjDf2 in Y75D11A, CAAGTGCCAAACTAGACTGCTC and TTCAAAACGCTACGCGATACCAG; fjDf3 in Y81B9A, AAATGCCCCTATCTCACAGTGG and GACTGCTAGAATCTGACTCGTC; fjDf4 in Y49A10A, CTCTTCCATTTCCAGTACAACCAG and GTTTCTATGGCTAGAGTCGTATGGTTAC. The PCR check can also be performed with the M13 reverse primer and the right-side primer. The e879 mutation can be checked by PCR with the following primers: AAATCAGGAGTGGGCATCAG and GGGAAGATTCCGATGAGTGA, followed by digestion with MvaI. The wild-type him-1 gene contains an MvaI site within its PCR region, while the e879 allele does not. Reference: Tabara H, et al. (2023) A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. EMBO J, e105002.
Proper citation: RRID:WB-STRAIN:WBStrain00054550 Copy
http://www.wormbase.org/db/get?name=WBStrain00054555
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: lgc-49(tm6556); zwEx175[Pflp-18::loxP::LacZ::STOP::loxP::mCherry::SL2::GFP, Pgpa-14::Cre]
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054555 Copy
http://www.wormbase.org/db/get?name=WBStrain00054556
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: acc-3(ok3450); zwEx175[Pflp-18::loxP::LacZ::STOP::loxP::mCherry::SL2::GFP, Pgpa-14::Cre]
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054556 Copy
http://www.wormbase.org/db/get?name=WBStrain00054540
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: cec-4(ok3124) him-8(e1489) IV
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054540 Copy
http://www.wormbase.org/db/get?name=WBStrain00054541
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: cec-5(tm6207) him-8(e1489) IV
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00054541 Copy
http://www.wormbase.org/db/get?name=WBStrain00054542
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017990(cec-4)|WBGene00017993(cec-5)|WBGene00021913(cec-8)
Genomic Alteration: WBGene00017990(cec-4), WBGene00017993(cec-5), WBGene00021913(cec-8)
Availability: unknown
References:
Synonyms: cec-8(fj63) III; cec-4(ok3124) cec-5(fj58) IV.
Notes: Maintain at 20C or lower. cec-8; cec-4 cec-5 triple mutants exhibit partial sterility and no significant defects in chromosome segregation. The chromodomain proteins CEC-5, CEC-4, and CEC-8 are phylogenetically similar to each other. The deletions can be detected by PCR with the following primers: cec-8(fj63): GCTGTATAATACTCACTATGTC and TCCAGCTCTGTAACCTTGAA; cec-4(ok3124): CAATTAAAATGCCAGTGCGA and TTTAGGATGCATTATGGGGC; cec-5(fj58): GCAAAGAAATCATCCGGTAGTG and CTTTGTAGCAACAGGCTCCTC. Reference: Tabara H, et al. (2023) A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. EMBO J, e105002.
Proper citation: RRID:WB-STRAIN:WBStrain00054542 Copy
http://www.wormbase.org/db/get?name=WBStrain00054548
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012802(set-25)|WBGene00019883(met-2)
Genomic Alteration: WBGene00012802(set-25), WBGene00019883(met-2)
Availability: unknown
References:
Synonyms: met-2(ok2307) set-25(n5021) III.
Notes: Maintain at 20C or lower. The met-2 set-25 double mutant exhibits partial sterility and no significant defects in chromosome segregation. MET-2 and SET-25 are the methyltransferases responsible for histone H3K9me2 and H3K9me3. The deletion mutations can be checked by PCR with the following primers: met-2(ok2307), GGTTGATGCGGAGAAGACTG and AATGGATTCGGTGCTTCGTG; set-25(n5021), GAGCCCGTGCCACAGAGTAG and CCTAGAGCGATGTCCTTGATGG. This strain was used as a negative control in the immunodetection of H3K9me2.
Proper citation: RRID:WB-STRAIN:WBStrain00054548 Copy
http://www.wormbase.org/db/get?name=WBStrain00054545
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: unknown
References:
Synonyms: fjDf1 fjDf2 fjDf3 fjDf4 X.
Notes: CeRep55 quadruple deletion: fjDf1 (also known as fj115); fjDf2 (aka fj85); fjDf3 (aka fj123); fjDf4 (aka fj120) X. This strain lacks four major clusters of CeRep55 repeats on the X chromosome. The condensation of unpaired X chromosomes in male testes is insufficient. CeRep55 is a class of minisatellite sequences consisting of a 27-nt tandem repeat that is present on all chromosomes. Some CeRep55 clusters express long non-coding RNAs and small RNAs. Each of the four deletion sites was designed to acquire a sequence tag (TGTACAGGAAACAGCTATGACC; similar to M13 reverse) instead of the CeRep55 tandem repeats. The deletions of CeRep55 clusters can be checked by PCR with the following primers: fjDf1 in Y73B3A, CAACCTGACTCTCGCCAAGAC and GGAGAAGTAGGCGTGTCAGTTA; fjDf2 in Y75D11A, CAAGTGCCAAACTAGACTGCTC and TTCAAAACGCTACGCGATACCAG; fjDf3 in Y81B9A, AAATGCCCCTATCTCACAGTGG and GACTGCTAGAATCTGACTCGTC; fjDf4 in Y49A10A, CTCTTCCATTTCCAGTACAACCAG and GTTTCTATGGCTAGAGTCGTATGGTTAC. The PCR check can also be performed with the M13 reverse primer and the right-side primer. Reference: Tabara H, et al. (2023) A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. EMBO J, e105002.
Proper citation: RRID:WB-STRAIN:WBStrain00054545 Copy
http://www.wormbase.org/db/get?name=WBStrain00054547
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00007297(vsra-1)|WBGene00017641(csr-1)
Genomic Alteration: WBGene00007297(vsra-1), WBGene00017641(csr-1)
Availability: unknown
References:
Synonyms: vsra-1(tm1637) I; csr-1(fj54)/tmC5 [F36H1.3(tmIs1220)] IV.
Notes: Sterile csr-1 allele balanced over tmC5 labelled with Venus. Heterozygotes are wild-type with somewhat dimmer Venus signal and segregate WT Venus(+) heterozygotes, Mec Unc Venus(+) tmC5 homozygotes, and non-Venus csr-1(fj54) homozygotes (sterile, but some animals lay a small number of dead eggs). Pick wild-type Venus(+) and check for proper segregation of progeny to maintain. Homologous pairing and unpaired silencing of meiotic chromosomes are inaccurate in homozygous tm1637; fj54 double mutants. The vsra-1 mutation enhances the defects caused by the csr-1 mutation. The fj54 deletion causes a frame-shift to stop the translation of both PAZ and Piwi domains. tm1637 can be detected by PCR with the following primers: AAGCAGTTCTTCAAGACTGGTC and TTGTCCACTCGCACTTTGTG. The fj54 deletion can be checked by PCR with the following primers: AAGAAATACCAATGCGGAGGCA and TTCACGGCTCTTTGCAGTTTCA. vsra-1 is also known as csr-2/C04F12.1. Reference: Tabara H, et al. (2023) A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. EMBO J, e105002.
Proper citation: RRID:WB-STRAIN:WBStrain00054547 Copy
http://www.wormbase.org/db/get?name=WBStrain00054646
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001804(gur-3)
Genomic Alteration: WBGene00001804(gur-3)
Availability: unknown
References:
Synonyms: gur-3(ok2245) X; wtfIs5.
Notes: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons in a gur-3(ok2245) mutant background. Derived from parental strain AML14 by integration of wtfEx4. Reference: Gauthey W, et al. Curr Biol. 2024 Jan 8;34(1):R14-R15. doi: 10.1016/j.cub.2023.10.043. PMID: 38194919.|"wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons. Derived from AML14 by integration of wtfEx4. Reference: Nguyen JP, et al. PLoS Comput Biol. 2017 May 18;13(5):e1005517."
Proper citation: RRID:WB-STRAIN:WBStrain00054646 Copy
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