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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rbp3[3’neo3,∆::GFP]/rbp3[3’neo3,∆::GFP];mpr1-1/mpr1-1; chx1-1/chx1-1 (mp-?, cy-?, ?)
Affected Genes: RPB3 (TTHERM_00193849)
Genomic Alteration: Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous
Availability:
References:
Synonyms:
Notes: Homozygous heterokaryon of ∆RPB3 (=cnjc). B2086 X Cu428 background, can mate with he∆ccg+-4-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02653 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Same as B2086-XS49 (15) but diff. cell line.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02136 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445D]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D, some have neo1 replacing coding
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pD445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445D. Partial replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02772 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-1/janA-1; disA-1/disA-1 (janA-1; disA-1; janA, disA, IV)
Affected Genes: JANA (janusA), DISA (disorganizedA)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Additive expression:the janus phenotype is superimposed upon a disorganized cortical landscape. It is uncertain whether or not eja1-1 is present in this stock.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01569 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX1/CHX1; mpr1?/mpr1? (mp-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
Availability:
References:
Synonyms:
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1 G1 14B.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02812 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ S134A]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
References:
Synonyms:
Notes: It is somatic rescue of strain of HTA1,HTA2 double knockout by HTA1 S134A mutation.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02384 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (chx1-1; cy-r, ory-r, tax-s, VII)
Affected Genes:
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00481 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU-HA-TAP]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
Availability:
References:
Synonyms:
Notes: Rescue of GTU05XGTU06 knockout heterokaryons with pTTMG-HA TAP. GTU1 with HA+TAP tag into MTT1 locus. MTT1 promoter, grow with 0.2ug/ml CdCl in 1xSPP.
5’MTT1-GTU-HA-TAP-MTT1 3’
TAP tag from Eric Phzicky is:
GTU-HA-6XHis-3Cprotease cleavage-S.Aureus protein A. Constuct pTTMG-HA used to rescue GTU5 xGTU06 KO heterokaryons.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02146 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2; bcd1-1/bcd1-1 (hpo1-2; bcd1-1; hpo1, bcd1, IV)
Affected Genes: HPO1 (hypoangular1), BCD1 (broadened cortical domains1)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: hpo1-2 strongly antagonizes the broadening of CVP domains brought about by bcd1-1; the resulting phenotype is a compromise between the broadening of the CVP domains brought about by bcd1 and the narrowing of these domains generated by hpo1
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01577 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1::neo2/gtu1::neo2;Chx1/Chx1; Mpr?/Mpr? (MTT1/GTU1[HA]; gtu1::neo2/gtu1::neo2; pm-r, BTU1 has(E431,435D), BTU2 is replaced by bsr1, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 gene replaced by neo2 cassette Macronucleus: HA tagged GTU1 gene into the MTT1 coding region
Availability:
References:
Synonyms:
Notes: Progeny of GTUKO5 x GTUKO6 mating, rescued by biolistic transformation with pTTMG-HA ( GTU-HA fusion driven by MTT1 promoter at the MTT1 locus). Needs 0.1μg/ml to 0.5 μg/ml Cd to grow, depending on the growth medium. Can cross with cTTMG-HA#15 or #14
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01216 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaE-1/cdaE-1 (cdaE-1, V)
Affected Genes: CDAE (cell division arrest E). (Originally published as mo8).
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Incomplete fission-zone formation with arrest in late-cytokinesis, with the new oral apparatus often abnormal and frequently wedged within the arrested furrow (39°). Fairly high lethality in crosses. Clear-cut temperature-sensitive expression: no expression at 28°, penetrance high but not 100% at 39° C.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01457 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946bp immediately upstream of ATG--GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: BTU1 locus replaced by far 5’BTU1 5’--neo3--CDC2 5’ flanking 946 bp immediately upstream of ATG--GFP coding--BTU1 3’
Availability:
References:
Synonyms:
Notes: Complete somatic transformant of CU522 cells. Construct was pBBN-CDC2: BTU1 5’(600bp from400-1000bp upstream of BTU1 ATG)--Neo3--CDC2 5’flanking 946 bp immediately upstream of ATG--GFP coding- -1.4 Kb BTU1 3â€Â. BTU1 promoter was deleted.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02823 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI11/twi11[5’neo3,HAn];MPR1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: HA tagged TWI11
Availability:
References:
Synonyms:
Notes: heterozygote HA-tagged TWI11 heterokaryon. HA coding sequences was inserted just after the start codon of TWI11. Neo3 cassette was inserted to upstream of TWI11
mates with HA-TWI11 B1-2-3.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02797 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-2/mat1-2 (mat1-2, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: All three B2086 clones were used extensively for crosses in the Frankel lab in the late 1980's and 1990's.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01625 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01869 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (rpc5[3’neo3,HAc]; pm-r, VII)
Affected Genes: RPC5 (TTHERM_00094210)
Genomic Alteration: Macronucleus: HA tagged RPC5
Availability:
References:
Synonyms:
Notes: All Somatic copy of RPA5 was replaced with RPA5-HA construct, neo3, CU428background. RPA5 is the fifth largest subunit of pol I and III.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02718 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01909 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaC-2/cdaC-2 (cdaC-2, VII)
Affected Genes: CDAC (cell division arrest C) Originally published as mo3b
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Failure to complete cytokinesis accompanied by cell elongation (>36o C). Located on chromosome 5. Allelic to cdaC-1 (IA-119). Expresses 100% fission blockage at temperatures of 36o C. and higher.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01511 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaA-2/cdaA-2 (cdaA-2, III)
Affected Genes: CDAA (cell division arrest A) Originally published as mo1b
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Partial failure to form a fission zone (39o C.) Fully penetrant at 39o C, but expressivity is far weaker than that of cdaA-1 at 39o, and instead resembles that of cdaA-1 at 35o C: dorsal ciliary rows remain continuous while ventral rows become subdivided, and a partial division furrow forms. Allelic to cdaA-1 (IA-104). The published phenotypic analysis was carried out on a different homozygous cdaA-2/cdaA-2 clone that is now extinct.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01510 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (CHX1; cy-s, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Cycloheximide resistance functional heterokaryon.
Proper citation: RRID:TSC_SD00701 Copy
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