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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1; tyrC3/tyrC3 (gal1-1; tyrC3; gal-r; tyr auxotroph, II)
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Genomic Alteration:
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Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01500 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
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Notes: A strain star line. Lacks a genetically functional micronucleus.
Proper citation: RRID:TSC_SD00014 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/CHX1; MPR1/mpr1-1; H1(neo)/+ (chx1-1/CHX1; MPR1/mpr1-1; cy-r, mp-r, pm-r, IV)
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Proper citation: RRID:TSC_SD00258 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: DCL1/dcl1[∆?::neo3] (DCL1/DCL1; pm-s, mp-?, ?)
Affected Genes: DCL1 (TTHERM_00284230)
Genomic Alteration: Micronucleus: heterozygous neo3 KO of DCL1,
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Notes: heterozygous heterokaryon of ∆DCL1 progeny of B2086xCU428, can mate with ∆SAI 2 g-1-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02836 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1[C3]-1/chx1[C3]-1 (CHX1[C3]; cy-s, I)
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Proper citation: RRID:TSC_SD00662 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD00945 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2R [B or C3] (pmr1-1; cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00396 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; Uni 2 (pmr1-1; cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00274 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 5 (CHX1; cy-s, VI)
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Proper citation: RRID:TSC_SD01128 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 3 [B or C3] (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00318 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 3 (MPR1; mp-s, VII)
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Proper citation: RRID:TSC_SD00835 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2] (?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 replaces coding of HTB1, HTB2
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Notes: HTB1/HTB2 double KO heterokaryons; Has different mating type from B2B4 and B4A6. Same mating type with B3D3 and D2B3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02063 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (tcd2[∆?::neo4]; pm-r, II)
Affected Genes: CHD4 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Part of 5’ flanking and coding region of TCD2 replaced by neo4 cassette.
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Notes: Somatic knockout strains of TCD2 affect Methylation of H3K27.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01772 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00289 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K33Q,K38Q,K40Q,K50Q,K51Q), GFPc]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to alanine (charge patch) and 5 other sites to glutamine, c-terminal GFP.
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Notes: Complete somatic tranformant of CU428 cells with pQ5A5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines,c-terminal GFP, neo 2 cassette in 3’ flanking region for selection. See paper.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02505 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (giw1[3’neo3,HAc]; p115:: p115-HA-neo3, pm-r
HA tagged GIW1p, VI)
Affected Genes: p115, GIWI (TTHERM_01276320 )
Genomic Alteration: Macronucleus: All GIW1 genes replaced by c-terminal HA tagged version with neo3 in 3’ flanking region
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Notes: All p115 copy in Mac was replaced with p115-HA-neo3 construct in CU427.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01819 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 3R (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00291 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-126; Basal body disorganization and stability defects (TS), ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-126
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Notes: Alanine scanning allele rescue of cen1∆ - Cen1 R126A, E127A, E130A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03281 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Availability:
References:
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Notes: somatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02232 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj2-1/cnj2-1 (CNJ2; PMR1; pm-s, IV)
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Proper citation: RRID:TSC_SD00211 Copy
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