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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT3/MAT3 (CHX1[C3];pmr1-1; MAT3; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01271 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01959 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2,∆,HAHAn(::TCD3)]; pm-r, VII)
Affected Genes: HPL4 (TTHERM_00585180)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD3, neo2 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Availability:
References:
Synonyms:
Notes: HA2-TCD2 overexpession from MTT1 locus, neo 2 upstream. HA-Tcd3p localizes to old mac and new mac.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02498 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Willard Pond in Antrim, NH (lat. 43 01.105, long. -72 01.262, elevation 1069 ft, nearest road is SR123, Willard Pnd Rd).
Geographical area: New England
Collection number: 1179
Has a micronucleus.
Proper citation: RRID:TSC_SD01561 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1[E445D]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D,
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pD445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445D. Full replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02771 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-1/janA-1; eja1-1/eja1-1; chx1-1/chx1-1 (janA-1; eja1-1; chx1-1; janA, eja1, cy-r, III)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus A); CHX1
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A mutation that causes the dorsal cell surface to be converted to a partial mirror-image of the ventral surface. Probably very similar to IA-220.
Proper citation: RRID:TSC_SD00631 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01961 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ubc2[∆::neo2]/ubc2[∆::neo2] (?)
Affected Genes: UBC2 (TTHERM_00550720)
Genomic Alteration: Micronucleus: Neo2 KO of UBC2
Availability:
References:
Synonyms:
Notes: UBC2 KO homozygous heterokaryon. Mates with ∆UBC2gl 1B2.8 and 1B2.10.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02536 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: eri1[∆::neo3]/eri1[∆::neo3]; mpr1-1/mpr1-1 (eri1[∆::neo3]; pm-r, ?)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: ERI1 coding replaced by neo3 Macronucleus: ERI1 coding replaced by neo3
Availability:
References:
Synonyms:
Notes: homozygous Er1 ko homokaryon ‘a’ can be mating with homozygous Er1 ko homokaryon ‘d’; coding sequences were replaced by Neo 3. Developmental delay.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02778 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1L (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00753 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BLT11-4288,BTU1429-443)], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pBbc-8 construct. BLT1(AA 1-428) with BTU1 at C-terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02261 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BLT11-4288,BTU1429-443)], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pBbc-8 construct. BLT1(AA 1-428) with BTU1 at C-terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02262 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2, ∆::(HHT3,GFPc)]/hht2[3’neo2, ∆::(HHT3,GFPc)] (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)], ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: C-terminal GFP- tag of HHT3, into HHT2 locus
Availability:
References:
Synonyms:
Notes: germline H3.3 +GFP at H3.2, heterokaryon. lnon-star side of H3.3+GFP germline replacement at H3.2 locus
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02303 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01974 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: bre1-1[∆::neo2]/bre1-1[∆::neo2]; MPR1-?/MPR1-? (mp-r, VI)
Affected Genes: BRE1 (TTHERM_00790530)
Genomic Alteration: Micronucleus: BRE1 homozygous for neo2 KO
Availability:
References:
Synonyms:
Notes: BRE1 KO heterokaryon;Made with B*VII
Mates with h∆BRE1(neo2)-A1E1
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02309 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1),HAc]; tax-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: GTU1 with c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
Availability:
References:
Synonyms:
Notes: pBTGBHA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 with HA tag under control of MTT1 promoter.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02306 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01187 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (hht1[∆::neo2]; hht1[∆::neo2]; HHF2;
hht2[K27R]; pm-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: WT HHF2 and mutated HHT2 with K27R
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 KO stains were mated and rescued with wt HHF2 gene/HHT2 gene with K27R mutation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02310 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01103 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00896 Copy
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