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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01079 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (twi1[3’neo3, FLAG-HAn]; pm-r, VI)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Availability:
References:
Synonyms:
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU427 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02608 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (HHF1;HHT1;HHF2/HHT2; mpr1-1; mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #7 strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01361 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon of ∆TWI1 progeny of hehe∆TWI1 F3-1 x CU427; assorted to pm-s; can mate with c5-2, c6-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02850 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1,T146G),HAc]; tax-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
Availability:
References:
Synonyms:
Notes: pT146G HA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 (T146G) with HA tag under control of MTT1 promoter. #1 and #5 are two separate single cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01365 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (cnjB[5’neo3,HAn]; pm- r, HA tagged CnjB, II)
Affected Genes: nnjB (TTHERM_01091290)
Genomic Alteration: Macronucleus: cnjB[5’neo3, HAn]
Availability:
References:
Synonyms:
Notes: Somatic HA-tagged Cnj-B. fully assorted. Mates with HA- CNB CU428 #2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01890 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HA-His-n]; Mac transformant, HA-His tagged hv2 gene
pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA-His- tag of HHT3, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: HA-His-tagged hv2 transformant. Checked by western blot. HA-His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01891 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Availability:
References:
Synonyms:
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01931 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pgp1[∆?::neo3]/pgp1[∆?::neo3] (pm-, ?)
Affected Genes: PGP1
Genomic Alteration: Micronucleus: neo3 into PGP1 locus
Availability:
References:
Synonyms:
Notes: Possible poly glycine protein gene was knocked out with neo3 cassette. This is a PPG1 KO heterokaryon with homozygous mic KO and mac assorted to wt.This strain can mate with PPG1∆4 cells. Final name of gene is PGP1when published.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02501 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, V)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01657 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2] /gtu1[GTU1,T270A,Y272A ]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A, Y272A. Not sure if complete replacement.
Availability:
References:
Synonyms:
Notes: pT270Y272 rescued GTUKO 5x6 progeny having mutation T270A,Y272A in GTU1 in GTU1 locus. Cell line #5 can be crossed with cell line #6.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02193 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T270A, Y272A)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pR285D291 rescued GTUKO 5x6 progeny having mutation @ T270,Y272-AA in GTU1-1 gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus.
#3 can be crossed with #6.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02194 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG B KO with neo (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01380 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Availability:
References:
Synonyms:
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02472 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF1; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF1
Availability:
References:
Synonyms:
Notes: EF-hand mutant allele rescue of cen1∆ - D36A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03284 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4] (drh1[∆?::neo4 ] Neo in 5’end of gene,some coding remains; pm-r, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 Macronucleus: neo4
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ D3-11F.
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Homozygous homokaryon. Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
Needs Cd for pm resistance.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02598 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];mpr1-1; MPR1[C3]; MAT2; cy-s, mp-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01267 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (Mac∆TWI1::neo3, twi1[∆::neo3]; mpr1-1; pm-r, mp-r, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: Coding region of TWI1 replaced by neo3
Availability:
References:
Synonyms:
Notes: B2086 X CU428 were transformed with TWI1 KO construct (whole coding was replaced by MTT-neo-BTU) and endogenous TWI1 gene was pushed out. Somatic transformant
this strain can mate with this strain can mate with CU428,cu427, TWI1-KO2,-KO 4.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02111 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (BTU1/btu1[∆,::neo1], BTU2; bsr-s, pm -r, ?)
Affected Genes: BTU1 (TTHERM_00348510) and BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: BTU1 coding replaced with neo1 cassette, BTU2 is blasticidin KO. Macronucleus: some BTU1::neo1 genes
Availability:
References:
Synonyms:
Notes: Obtained from Jacek 11/1/99. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO.
Mates with DB2A for heterokaryon rescue.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01943 Copy
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