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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1,HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
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Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region with HA tag .Progeny of GTUKO5x6, transformed with pTTMGHA construct (GTU coding, HA tag driven by MTT promoter) to rescue GTU KO heterokaryon. Can mate with cTTMG-HA #12 or #15.
Requires Cd to grow, use .25 to 1 ug/ml. GTU1-HA detected at protein level.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01341 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 4 (CHX1; cy-s, VI)
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Proper citation: RRID:TSC_SD01069 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2 (htax[∆::neo2]/htax[K123R]; hta1[∆::neo2]; pm-r, mp-r, II)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 KO of HTAX and HTA1 Macronucleus: Neo2 KO of HTA1, partial KO of HTAX some HTAX has K123R mutation
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Notes: New batch, rescue of h∆∆HTA-A1F14A1 and B1G6A
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02398 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (ATU1; mpr1-1; mp-r, VII)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo coding replaces ATU1 coding
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Notes: ATU knockout heterokaryon. Transferred many times before being frozen. Mates with AAKO2.7.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01901 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 1R [chx1-1/chx1-1[B] or CHX1/CHX1[C3]] (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00381 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2L [PMS/PMS B or PMR/PMR C3] (pmr1-1; cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00382 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA2 has neo2; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
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Notes: It is HTA1 WT somatic rescue strain of HTA1,HTA2 double knockout. Can mate with 1-3(2).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02168 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (wild type C3, V)
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Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Regenerated micronucleus from C3 368 by "star" mating. Non-star side maintained.
Proper citation: RRID:TSC_SD00026 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: [chx1-1/chx1-1[B] or CHX1/CHX1[C3]]; Uni 1 (cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00306 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01084 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
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Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01240 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01003 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; disA/disA (CHX1; cy-s, tr, VII)
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Proper citation: RRID:TSC_SD00155 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
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Proper citation: RRID:TSC_SD01095 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (IV)
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Proper citation: RRID:TSC_SD00283 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, ?)
Affected Genes: HHO1 (TTHERM_00823720)
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Notes: somatic gene replacement use A5 mutation to replace wild type HHOI gene neo cassette is at 3’ flanking region
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02222 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2] (MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2]; mp-r, pm-r, ?)
Affected Genes: JMJ2 (TTHERM_00455500)
Genomic Alteration: Micronucleus: JMJ2 gene partially replaced by neo4-KanR2 cassette.
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Notes: The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01775 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; gal1-1/gal1-1; TS11/TS11 or ts11-1/ts11-1; Nulli 1L,2R,3,5 (CHX1; GAL1?; mpr1-1; ts11-1; cy-s, gal?, mp-r, ts, IV)
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Proper citation: RRID:TSC_SD00169 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; chx2-1/chx2-1; MPR1/MPR1 (CHX1; CHX2; cy-s, mp-r, ts, II)
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Proper citation: RRID:TSC_SD00047 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 1L (CHX1; cy-s, VI)
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Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00721 Copy
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