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https://www.thelabworldgroup.com/ge-typhoon-9410-microarray-imager
Variable mode imager that operates in three different modes, including phosphorescence, fluorescence, and chemiluminescence. Can scan in visible range using reflected light. Scanning resolution ranges from 1000um down to 10um. Scanning software is linked to ImageQuant TL 7.0 analysis software. ImageQuant is capable of quantifying Typhoon images such as those from Northern or Western blots. Images can be saved in various formats and imported into Powerpoint slides. Instrument has three excitation lasers (532nm, 633nm, 488nm). Instrument utilizes 536SP, 670BP, 520BP emission filters for fluorescence. For DNA, RNA and protein samples you may choose from: storage phosphor auto-radiography, direct blue-excited fluorescence (457-488 nm), direct green-excited fluorescence (532 nm), direct red-excited fluorescence (633 nm), and chemiluminescence.
Proper citation: GE Healthcare 9410 Variable Mode Imager (RRID:SCR_018047) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/products/leica-rm2265/
Automated rotary microtome that sections tissue samples. Specimen feed system with zero-backlash and maintenance-free cross roller guides and stepper motor operated coarse feed system are located in dust-proof plastic housing. Equipped with safety handwheel with handle that can be centered, as well as mechanical handwheel lock. In manual operation retraction can be adjusted. In motorized operation retraction value varies with selected sectioning speed.
Proper citation: Leica Biosystems RM2265 Fully Automated Rotary Microtome (RRID:SCR_018041) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/leica-rm2125-rts/
Manual rotary microtome that sections tissue samples. Features include precise feed mechanism, powerBase that ensures stable operation for vibration-free sectioning, advanced knife holder and specimen clamp securely align cutting edge to block, user-selectable coarse feed wheel turn direction and retraction functions, hand wheel, in-built safety guards and rounded shape with arm rests for fatigue-free sectioning,quick-exchange specimen clamp and orientation system for object head allow rapid set up for any block, and two trim steps.
Proper citation: Leica RM2125 RTS Manual Microtome (RRID:SCR_018040) Copy
https://www.thermofisher.com/order/catalog/product/313001R#/313001R
Genetic analyzer can run sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening. Maintenance time is reduced by eliminating manual syringe washing and filling with automated polymer delivery. Can increase data quality for sequencing and fragment analysis applications by longer read length, and higher resolution with shorter run times. Uses 96- or 384-well plates with four capillary array.
Proper citation: Applied Biosystems 3130 Genetic Analyzer (RRID:SCR_018046) Copy
https://timothyspringer.org/files/tas/files/biacore3000-instrument.pdf
Biacore 3000 processing unit is an established, label-free system for detailed studies of biomolecular interactions. The system delivers comprehensive characterization of the interaction, answers questions about the rate constants, affinity, specificity, and determines the active concentration of components. The ability to recover and transfer interaction partners directly to MALDI targets for identification and further characterization makes the system highly applicable to fast identification of unknown interactants.
Proper citation: Biacore 3000 Real Time Biomolecular Interaction Analyzer (RRID:SCR_018044) Copy
Bioanalyzer system is automated electrophoresis tool that provides an analytical evaluation of various samples types in many workflows, including next generation sequencing NGS, gene expression, biopharmaceutical, and gene editing research. Digital data is provided in timely manner and delivers assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for accurate result, and data may be exported in many different formats.
Proper citation: Agilent 2100 Bioanalyzer Instrument (RRID:SCR_018043) Copy
http://www.thunderbiosci.co.kr/wp-content/uploads/2017/07/Apollo-324_brochure.pdf
Compact benchtop platform that enables rapid and full walk-away automation of next generation sequencing applications. Paired with PrepX reagents, Apollo 324 line offers complete sample-toanswer solution for NGS library prep. Apollo 324 automated protocols and PrepX reagent kits combine liquid handling and proven chemistries to produce high quality libraries for all major NGS platforms. System provides complete, walkaway solution using validated protocols and chemistries that produce consistent libraries for DNA-seq, RNA-seq, and ChIP-seq applications.
Proper citation: Wafergen Apollo 324 NGS Library Prep (RRID:SCR_018027) Copy
https://www.nanostring.com/products/ncounter-analysis-system/ncounter-systems-overview/
System includes hardware and software. Options include nCounter SPRINT,nCounter MAX or nCounter FLEX. Platform used for multiplex analysis of up to 800 RNA, DNA, or protein targets. System can be combined with GeoMx Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Proper citation: NanoString nCounter Analysis System (RRID:SCR_021712) Copy
CGB offers range of genomic services, including high-throughput DNA/RNA extraction, library preparation, next-generation sequencing, and bioinformatic analysis.
Proper citation: Indiana University Center for Genomics and Bioinformatics Core Facility (RRID:SCR_017165) Copy
https://pathology.med.upenn.edu/research/core-resources/tumor-tissue-biospecimen-bank
Centralized biorepository of human biosamples. TTAB is responsible for the collection, processing, and storage of human blood, fluid, and tissue at the University of Pennsylvania Health System. TTAB has a collection bench within Surgical Pathology Suite at Hospital of University of Pennsylvania (HUP).� Our collection bench sits adjacent to frozen section teams managing clinical sample collection and allowing for tight integration of tissue sample collection with clinical pathology teams.
Proper citation: University of Pennsylvania Perelman School of Medicine Tumor Tissue Biospecimen Bank Core Facility (RRID:SCR_022430) Copy
https://www.med.upenn.edu/ifi/cytofservicecenter.html
CyTOF�enables multi-parametric high-dimensional single�cell analysis�of more than 40 markers per cell, with�minimal background and compensation�issues.�Core�offers variety of�CyTOF-related services including�reagent distribution, consultation,�antibody conjugation, and data acquisition.�
Proper citation: University of Pennsylvania Perelman School of Medicine IFI CyTOF Service Center Core Facility (RRID:SCR_022410) Copy
BioBank supports researchers by providing centralized access to large number of annotated blood and tissue samples.
Proper citation: University of Pennsylvania Perelman School of Medicine Penn Medicine BioBank Core Facility (RRID:SCR_022415) Copy
http://tools.thermofisher.com/content/sfs/manuals/nd-1000-v3.8-users-manual-8%205x11.pdf
Spectrophotometer for measurement and analysis of 1 ul samples with high accuracy and reproducibility. Full spectrum from 220nm to 750nm spectrophotometer utilizes patented sample retention technology that employs surface tension alone to hold sample in place. No need for cuvettes. Has capability to measure highly concentrated samples without dilution.
Proper citation: Thermo Scientific Nanodrop 1000 Spectrophotometer (RRID:SCR_016517) Copy
Benchtop genetic analyzer. Electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow.
Proper citation: Agilent 4200 TapeStation System (RRID:SCR_018435) Copy
http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf
Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.
Proper citation: Applied Biosystems GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy
Provides services for USED lab instrument repairs.Recertified laboratory equipment commercially available. Comes fully re-certified to manufacturers standards with 90 day warranty.
Proper citation: Certified Genetool, Inc. (RRID:SCR_018678) Copy
Center coordinates ideas and investments to advance and democratize access to Stanford cores, instrumentation, scientific and technical staff, allowing users to perform innovative, rigorous experiments.
Proper citation: Stanford University Community of Shared Advanced Research Platforms (RRID:SCR_022986) Copy
https://www.fredhutch.org/en/research/shared-resources.html
Through Shared Resources, investigators in Fred Hutch/University of Washington Cancer Consortium and external academic and industry organizations can access services tailored to their specific research goals, including novel assay development or early access to new technologies.
Proper citation: Fred Hutchinson Cancer Center Shared Resources (RRID:SCR_022607) Copy
Provides genomics and molecular biology services for University of Delaware research groups and outside users.Supports genomic research through established expertise with genomics technologies.
Proper citation: University of Delaware Sequencing and Genotyping Center Core Facility (RRID:SCR_012230) Copy
https://moffitt.org/research-science/shared-resources/molecular-genomics/
Molecular Genomics Core Facility at Moffitt Cancer Center in Tampa, FL offers services to assess gene expression, epigenetic modification and sequence variation. Performs bulk and single cell RNA and DNA sequencing on Illumina platform, NanoString nCounter, GeneChip and Illumina array services.Offers services to discuss your project and timeline for your project.
Proper citation: Moffitt Cancer Center Molecular Genomics Core Facility (RRID:SCR_012333) Copy
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