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Species: Saccharomyces cerevisiae
Genetic Insert: FLP1
Vector Backbone Description: Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and
CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids.
Proper citation: RRID:Addgene_160380 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SKI1
Vector Backbone Description: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160382 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPA1
Vector Backbone Description: Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed)
Proper citation: RRID:Addgene_102614 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: APEX2-NES
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102951 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: bPAC
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103017 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Csy4
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103139 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103140 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNAs
Vector Backbone Description: Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103141 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100989 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100986 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RP51A
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100991 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HH-gRNA-HDV targetting SeILV6 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
Vector Backbone Description: Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101168 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100002 Copy
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