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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGAL-flp(H305L)-NAT Resource Report Resource Website |
RRID:Addgene_160380 | FLP1 | Saccharomyces cerevisiae | Ampicillin | PMID: | NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. | Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | H305L | 2022-12-10 12:02:51 | 0 |
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pGAL-SKI1-NAT Resource Report Resource Website |
RRID:Addgene_160382 | SKI1 | Saccharomyces cerevisiae | Ampicillin | PMID: | Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-12-10 12:02:51 | 0 | ||
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p11d-sctRPA Resource Report Resource Website |
RRID:Addgene_102614 | RPA1 | Saccharomyces cerevisiae | Ampicillin | PMID:9730822 | RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed) | Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:17 | 0 | |
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pG23A Resource Report Resource Website |
RRID:Addgene_102884 | Cdc14/mCherry-AID(71-114)-NatMX | Saccharomyces cerevisiae | Ampicillin | PMID:27989441 | This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast. A characterization guide can be found here: https://www.nature.com/articles/s41598-017-04791-6 Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper: http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2 | Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:21 | 0 | |
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pJH124 Resource Report Resource Website |
RRID:Addgene_102951 | APEX2-NES | Saccharomyces cerevisiae | Ampicillin | PMID:27274088 | Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:23 | 0 | ||
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pHES609 [prNOp7-PBAC] Resource Report Resource Website |
RRID:Addgene_103017 | bPAC | Saccharomyces cerevisiae | Ampicillin | PMID:28035051 | Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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p413_Csy4NLS Resource Report Resource Website |
RRID:Addgene_103139 | Csy4 | Saccharomyces cerevisiae | Ampicillin | PMID:29161506 | Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:26 | 0 | ||
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dCas9v2 Resource Report Resource Website |
RRID:Addgene_103140 | dCas9-VPR | Saccharomyces cerevisiae | Ampicillin | PMID:29161506 | Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | Removed scaffold gRNA from original plasmid | 2022-04-22 03:15:26 | 0 | |
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pCRA03 Resource Report Resource Website |
RRID:Addgene_103141 | gRNAs | Saccharomyces cerevisiae | Ampicillin | PMID:29161506 | Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:26 | 0 | ||
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Rp51a Hyperstabilized 5'SS Resource Report Resource Website |
RRID:Addgene_100988 | RP51A | Saccharomyces cerevisiae | Ampicillin | PMID:28829039 | Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | 5'ss mut ATG/GTATGT -> AAG/GTAAGT | 2022-04-22 03:14:59 | 0 | |
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Rp51a_10bp 5'SS_No BP or pBP_pUC18 Resource Report Resource Website |
RRID:Addgene_100989 | RP51A | Saccharomyces cerevisiae | Ampicillin | PMID:28829039 | Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation | 2022-04-22 03:14:59 | 0 | |
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RP51A-NoBS-NoPseudoBS Resource Report Resource Website |
RRID:Addgene_100986 | RP51A | Saccharomyces cerevisiae | Ampicillin | PMID:28829039 | Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | BS mutated to GTTAGTG, pseudo BS mutated to GTTTGTG | 2022-04-22 03:14:59 | 0 | |
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Rp51a_SUS1 5'ss_No_BP_pBP-pUC18 Resource Report Resource Website |
RRID:Addgene_100990 | RP51A | Saccharomyces cerevisiae | Ampicillin | PMID:28829039 | Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation | 2022-04-22 03:14:59 | 0 | |
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Rp51a_SUS1 5'SS Resource Report Resource Website |
RRID:Addgene_100991 | RP51A | Saccharomyces cerevisiae | Ampicillin | PMID:28829039 | Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin | GGTAtGT->tGTAtGa 5'SS mutant | 2022-04-22 03:14:59 | 0 | |
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pUDP010 Resource Report Resource Website |
RRID:Addgene_101166 | HH-gRNA-HDV targetting SeILV6 in S. pastorianus | Saccharomyces cerevisiae | Ampicillin | PMID:29207996 | Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | ||
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pUDP012 Resource Report Resource Website |
RRID:Addgene_101167 | HH-gRNA-HDV targetting SeILV6 in S. pastorianus | Saccharomyces cerevisiae | Ampicillin | PMID:29207996 | Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | ||
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pUDP044 Resource Report Resource Website |
RRID:Addgene_101168 | polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus | Saccharomyces cerevisiae | Ampicillin | PMID:29207996 | Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | ||
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pDONR221-Sgd1 Resource Report Resource Website |
RRID:Addgene_100000 | Sgd1 | Saccharomyces cerevisiae | Kanamycin | PMID:29054886 | Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1). | Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:45 | 0 | |
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pACT2-Dhr1 Resource Report Resource Website |
RRID:Addgene_100003 | Dhr1 | Saccharomyces cerevisiae | Ampicillin | PMID:29054886 | Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1. | Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:46 | 0 | |
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pAS2-1-Dhr1 Resource Report Resource Website |
RRID:Addgene_100002 | Dhr1 | Saccharomyces cerevisiae | Ampicillin | PMID:29054886 | Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1. | Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:45 | 0 |
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