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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETtv-2in1-CN; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105119 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETvr-2in1-CC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105117 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETtv-2in1-NN; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105125 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETvr-2in1-NN; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105126 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETvr-2in1-NC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105123 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pZMU-Dest; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105089 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRETtv-2in1-NC; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_105122 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: The ccdB has an H55Q mutation that does not affect function.
Proper citation: RRID:Addgene_105338 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pFRm43GW; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_133748 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7495; Vector Backbone:pBSWHhc; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_133901 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153229 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153228 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153236 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ordon et al, 2017; Backbone Size:7000; Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153235 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Other, Gateway; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Other, Gateway; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Other, Gateway; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Species: Synthetic
Genetic Insert: RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
Vector Backbone Description: Backbone Marker:VIB, Ghent Univ, Karimi et al. Trends Plant Sci. (2002); Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: Gateway destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter. Use with Platinum Gate TALEN kit (Kit # 1000000043) and plasmids #158728-#158735.
Proper citation: RRID:Addgene_163145 Copy
Species: Other
Genetic Insert: pEC1.2::spCas9
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167686 Copy
Species: Other
Genetic Insert: PcUBQ4-2::asCpf1
Vector Backbone Description: Vector Backbone:pDe-CAS9; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
References:
Comments: In addition to Cas9 cassette, attR1-attR2 Gateway recombination sites together with ccdB cassette are present.
Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167685 Copy
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