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Species: Other
Genetic Insert: srRNA HFQ handle
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: HFQ handle based on sRNA MicC from E. coli (see Fig. 1). Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119630 Copy
Species: Synthetic
Genetic Insert: J23119MH_V07
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119598 Copy
Species: Other
Genetic Insert: 6803 cpcBAC1&C2 Down Flank
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Synechocystis sp. PCC 6803, sequence downstream of cpcBA operon. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119631 Copy
Species: Synthetic
Genetic Insert: J23119MH_V04
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119595 Copy
Species: Other
Genetic Insert: 6803 NS4 Up Flank
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Addgene QC NGS identified a sequence discrepancy in the plasmid insert. The depositing lab has confirmed that this should not affect plasmid function.
Synechocystis sp. PCC 6803, sequence upstream of slr0397. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119639 Copy
Species: Other
Genetic Insert: sacB
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Levansucrase expression cassette (CDS). Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119623 Copy
Species: Other
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Catalytically deactivated Cas9 (dCas9) (CDS). www.addgene.org/44249 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119621 Copy
Species: Synthetic
Genetic Insert: J23116MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119589 Copy
Species: Other
Genetic Insert: dCas9+deg-tag
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: dCas9 with a C-terminal protein degradation tag (CDS). Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119622 Copy
Species: Other
Genetic Insert: AbRChl
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41264; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: AbR cassette for Chloramphenicol.
Please note: due to an error in cloning, the 3’ overhang (GCTT) typically generated by BsaI is absent for this part, and has been changed to ATCT. Thus, this vector will not assemble as expected. The ABR cassette for Chloramphenicol is correct.
Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119627 Copy
Species: Other
Genetic Insert: AbRSpec
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41264; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: AbR cassette for Spectinomycin. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119625 Copy
Species: Other
Genetic Insert: AbREry
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41264; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: AbR cassette for Erythromycin. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119626 Copy
Species: Synthetic
Genetic Insert: J23119MH_V02
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119593 Copy
Species: Synthetic
Genetic Insert: J23119MH_V03
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119594 Copy
Species: Synthetic
Genetic Insert: J23118MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119591 Copy
Species: Synthetic
Genetic Insert: J23119MH_V01
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119592 Copy
Species: Synthetic
Genetic Insert: J23117MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119590 Copy
Species: Synthetic
Genetic Insert: Unmarked Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119612 Copy
Species: Synthetic
Genetic Insert: UP Flank Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119613 Copy
Species: Other
Genetic Insert: TrrnB
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: E. coli double terminator rrnB T1 and T7Te. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119611 Copy
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