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  • Organism:drosophila melanogaster (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pMT-ADARcd-E488Q-V5
 
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RRID:Addgene_139686 Adenosine deaminase acting on RNA Drosophila melanogaster Ampicillin PMID:29127211 Vector Backbone:pMT; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:26:40 0
pRD182 (Roc1b)
 
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RRID:Addgene_14823 Roc1b Drosophila melanogaster Ampicillin PMID: Backbone Marker:Promega; Backbone Size:2600; Vector Backbone:T7 IVT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:27:46 0
pRD183 (Roc2)
 
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RRID:Addgene_14824 Roc2 Drosophila melanogaster Ampicillin PMID: Backbone Marker:Promega; Backbone Size:2600; Vector Backbone:T7 IVT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:27:46 0
pRD60 (dE2F)
 
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RRID:Addgene_14820 E2F Drosophila melanogaster Ampicillin PMID: Dros14 dE2F clone flipped at EcoRI site; XhoI digestion gives small fragment--i.e. 5' end near KpnI site; contains entire 3' UTR from Genebank sequence including polyA. Backbone Marker:na; Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:27:46 0
pAc5.1B-EGFP-DmLSm1_A
 
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RRID:Addgene_145880 DmLSm1 Drosophila melanogaster Ampicillin PMID:17923697 Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin two silent mutations compared to the sequence given by NCBI: NM_137715.3 2022-04-22 03:27:30 0
pGC(2.1)-U6.3
 
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RRID:Addgene_170516 gRNAcore(2.1)-pU6.3 Drosophila melanogaster Kanamycin PMID:33782117 For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/ Backbone Size:2351; Vector Backbone:pDONR221; Vector Types:Other, PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP.; Bacterial Resistance:Kanamycin 2022-04-22 03:32:30 0
pTR(EF)-tRNA(Q)
 
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RRID:Addgene_170517 gRNAcore(EF)-tRNA(Q) Drosophila melanogaster Kanamycin PMID:33782117 For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/ Backbone Size:2352; Vector Backbone:pDONR221; Vector Types:Other, PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80; Bacterial Resistance:Kanamycin 2022-04-22 03:32:30 0
pUASTattB-2xFlag-mCherry-Msp300KASH
 
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RRID:Addgene_170807 2xFLAG/mCherry-Msp300KASH Drosophila melanogaster Ampicillin PMID:34022041 Plasmid first described in: Hall H., Medina P., Cooper D.A., Escobedo S.E., Rounds J., Brennan K.J., Vincent C., Miura P., Doerge R. and Weake V.M. (2017). Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics. 18(1):894. PMID: 29162050. Backbone Size:9355; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:32:35 0
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
 
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RRID:Addgene_163926 Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor Drosophila melanogaster Ampicillin PMID:28395731 To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence. pUASTLOTattB vector: Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development 141, 472–480 (2014). vhhGFP4 plasmid: Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K J Mol Biol. 2005 Sep 23; 352(3):597-607. mCherry: Clonetech Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin 2022-04-22 03:31:03 0
AByI
 
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RRID:Addgene_164153 Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right Drosophila melanogaster Ampicillin PMID:30079589 Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:31:08 0
AByB
 
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RRID:Addgene_164148 Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right Drosophila melanogaster Ampicillin PMID:30079589 Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:31:08 0
AByC
 
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RRID:Addgene_164149 Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right Drosophila melanogaster Ampicillin PMID:30079589 Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin 2022-04-22 03:31:08 0
OA-1043
 
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RRID:Addgene_164586 U6 and DR Drosophila melanogaster Ampicillin PMID:33616113 Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:31:15 0
pUASP Flag Rab32 CA
 
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RRID:Addgene_44834 Rab32 Drosophila melanogaster Ampicillin PMID: Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin Q79L (constitutive active) 2022-04-22 03:41:16 0
pUASP Flag Rab32 DN
 
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RRID:Addgene_44835 Rab32 Drosophila melanogaster Ampicillin PMID: Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin T33N (dominant negative) 2022-04-22 03:41:16 0
pUASP dsRed Rab27 WT
 
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RRID:Addgene_44832 Rab27 Drosophila melanogaster Ampicillin PMID:17409086 Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin 2022-04-22 03:41:16 0
pUASP dsRed Rab11 DN
 
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RRID:Addgene_44831 Rab11 Drosophila melanogaster Ampicillin PMID:17409086 Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin S25N (dominant negative) 2022-04-22 03:41:16 0
pUASP dsRed Rab11 WT
 
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RRID:Addgene_44829 Rab11 Drosophila melanogaster Ampicillin PMID:17409086 Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin 2022-04-22 03:41:16 0
pUASP dsRed Rab3 WT
 
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RRID:Addgene_44820 Rab3 Drosophila melanogaster Ampicillin PMID:17409086 Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Other, Drosophila; Bacterial Resistance:Ampicillin 2022-04-22 03:41:16 0
psiCheck-2-3 x mir-34
 
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RRID:Addgene_40831 three sites partially complementary to miR-34 Drosophila melanogaster Ampicillin PMID:22055293 The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC the triple(3x)-repeated sequence: 5'TGGCAGTGACCTTAGCATCAACAC-3' 3'ACCGTCACTGGAATCGTAGTTGTG-5' pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion. Backbone Marker:promega; Backbone Size:6273; Vector Backbone:psiCheck-2; Vector Types:Insect Expression, RNAi; Bacterial Resistance:Ampicillin 2022-04-22 03:40:12 0

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