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Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_114441 Copy
Species: Homo sapiens
Genetic Insert: PARK7C53A
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:Other, gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
References:
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115179 Copy
Species: Homo sapiens
Genetic Insert: PARK7E163K
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:Other, gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
References:
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115181 Copy
Species:
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Species: Arabidopsis thaliana
Genetic Insert: pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_115995 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116013 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116016 Copy
Species:
Genetic Insert: wbbL
Vector Backbone Description: Vector Backbone:pEXT21; Vector Types:; Bacterial Resistance:Spectinomycin
References:
Comments: Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function.
Proper citation: RRID:Addgene_113662 Copy
Species: Other
Genetic Insert: dspSix5
Vector Backbone Description: Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007)
Proper citation: RRID:Addgene_118013 Copy
Species: Arabidopsis thaliana
Genetic Insert: AIP2
Vector Backbone Description: Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: 28 C in GV3101 (pMP90) strain
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118687 Copy
Species: Arabidopsis thaliana
Genetic Insert: AIP2
Vector Backbone Description: Vector Backbone:pBA cMYC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: 28 C in GV3101 (pMP90) strain
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118667 Copy
Species: Arabidopsis thaliana
Genetic Insert: PHL2
Vector Backbone Description: Vector Backbone:pBA cMYC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: 28 C in GV3101 (pMP90) strain
Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications:
Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5
Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416
Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9
Proper citation: RRID:Addgene_118669 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112073 Copy
Species: Other
Genetic Insert: TagRFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112081 Copy
Species: Other
Genetic Insert: AtCAS9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9 vector from H. Puchta lab.
Proper citation: RRID:Addgene_112070 Copy
Species: Homo sapiens
Genetic Insert: Human MCM6
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_116951 Copy
Species: Other
Genetic Insert: ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_117236 Copy
Species: Other
Genetic Insert: SpCas9-p (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117521 Copy
Species: Other
Genetic Insert: SpCas9-h (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117520 Copy
Species: Other
Genetic Insert: SpCas9-p (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117522 Copy
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