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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCR8 LCOR Resource Report Resource Website |
RRID:Addgene_114441 | LCOR | Homo sapiens | Spectinomycin | PMID:29628311 | Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, Gateway Entry Cloning; Bacterial Resistance:Spectinomycin | 2022-04-22 03:18:39 | 0 | ||
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pENTR223-PARK7C53A Resource Report Resource Website |
RRID:Addgene_115179 | PARK7C53A | Homo sapiens | Spectinomycin | PMID:31536960 | Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation. | Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:Other, gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin | p.C53A | 2022-04-22 03:18:57 | 0 |
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pENTR223-PARK7E163K Resource Report Resource Website |
RRID:Addgene_115181 | PARK7E163K | Homo sapiens | Spectinomycin | PMID:31536960 | Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation. | Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:Other, gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin | p.E163K | 2022-04-22 03:18:57 | 0 |
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pMN19 Resource Report Resource Website |
RRID:Addgene_11572 | CaMV 35S enhancers | Spectinomycin | PMID:10759496 | Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. | Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:08 | 0 | ||
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pSW646 - pML1>> Resource Report Resource Website |
RRID:Addgene_115995 | pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR | Arabidopsis thaliana | Spectinomycin | PMID:30026289 | Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:15 | 0 | ||
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pVL47 - pSCR>> Resource Report Resource Website |
RRID:Addgene_116013 | pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR | Arabidopsis thaliana | Spectinomycin | PMID:30026289 | Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:15 | 0 | ||
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pVL94 - pOp4:VND7 Resource Report Resource Website |
RRID:Addgene_116016 | pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR | Arabidopsis thaliana | Spectinomycin | PMID:30026289 | Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:15 | 0 | ||
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pMF19 Resource Report Resource Website |
RRID:Addgene_113662 | wbbL | Spectinomycin | PMID:10574995 | Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function. | Vector Backbone:pEXT21; Vector Types:; Bacterial Resistance:Spectinomycin | 2022-04-22 03:18:22 | 0 | ||
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Six5 pGWB402 Resource Report Resource Website |
RRID:Addgene_118013 | dspSix5 | Other | Spectinomycin | PMID:29481865 | Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007) | Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:44 | 0 | |
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pER8 cHA-AIP2 Resource Report Resource Website |
RRID:Addgene_118687 | AIP2 | Arabidopsis thaliana | Spectinomycin | PMID:28400782 | 28 C in GV3101 (pMP90) strain Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 | Vector Backbone:pER8 cHA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:58 | 0 | |
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pBA cMYC-AIP2 Resource Report Resource Website |
RRID:Addgene_118667 | AIP2 | Arabidopsis thaliana | Spectinomycin | PMID:28400782 | 28 C in GV3101 (pMP90) strain Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 | Vector Backbone:pBA cMYC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:58 | 0 | |
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pBA cMYC-PHL2 Resource Report Resource Website |
RRID:Addgene_118669 | PHL2 | Arabidopsis thaliana | Spectinomycin | PMID:28400782 | 28 C in GV3101 (pMP90) strain Our work is based on genomic approaches in Arabidopsis thaliana and groups of genes are presented in all of the following publications: Pavicic Mirko (2019). Multifaceted roles of RING-type ubiquitin E3 ligases: reverse phenomic approaches. http://urn.fi/URN:ISBN:978-951-51-5483-5 Pavicic M., Mouhu K., Wang F., Bilicka M., Chovancek E., & Himanen K. (2017). Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis. Frontiers in Plant Science. doi.org/10.3389/fpls.2017.00416 Pavicic M., Wang F., Mouhu K., Himanen K. (2019). High throughput in vitro seed germination screen identified new ABA responsive RING-type ubiquitin E3 ligases in Arabidopsis thaliana. Plant Cell Tissue and Organ Culture. https://link.springer.com/article/10.1007/s11240-019-01700-9 | Vector Backbone:pBA cMYC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:58 | 0 | |
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pORFE0005 Resource Report Resource Website |
RRID:Addgene_112073 | AtDeadCAS9 D10A H840A without stop codon | Other | Spectinomycin | PMID: | BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis. | Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin | Plant codon optimized dCas9 D10A S. pyogenes | 2022-04-22 03:17:59 | 0 |
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pJPN0015 Resource Report Resource Website |
RRID:Addgene_112081 | TagRFP | Other | Spectinomycin | PMID: | BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis. | Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | BsaI and BpiI restriction sites have been removed from the sequence | 2022-04-22 03:17:59 | 0 |
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pORFE0001 Resource Report Resource Website |
RRID:Addgene_112070 | AtCAS9 | Other | Spectinomycin | PMID: | BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9 vector from H. Puchta lab. | Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin | Plant codon optimized Cas9 S. pyogenes | 2022-04-22 03:17:59 | 0 |
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pCDF-MCM4/6 Resource Report Resource Website |
RRID:Addgene_116951 | Human MCM6 | Homo sapiens | Spectinomycin | PMID:25648893 | Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function. | Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:28 | 0 | |
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pBECKP-spe Resource Report Resource Website |
RRID:Addgene_117236 | ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase | Other | Spectinomycin | PMID:30217854 | Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:32 | 0 | ||
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pEPOR0SP0013 Resource Report Resource Website |
RRID:Addgene_117521 | SpCas9-p (with stop codon) | Other | Spectinomycin | PMID:30811422 | Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint | Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:36 | 0 | |
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pICSL90005 Resource Report Resource Website |
RRID:Addgene_117520 | SpCas9-h (no stop codon) | Other | Spectinomycin | PMID:30811422 | Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint | Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:36 | 0 | |
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pEPOR0SP0009 Resource Report Resource Website |
RRID:Addgene_117522 | SpCas9-p (no stop codon) | Other | Spectinomycin | PMID:30811422 | Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint | Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin | 2022-04-22 03:19:36 | 0 |
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