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http://www.addgene.org/30173

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.

Proper citation: RRID:Addgene_30173 Copy   

•   Source: Addgene

http://www.addgene.org/30175

Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mOrange2. The mOrange2 ORF is present at bp# 5620-6330.

Proper citation: RRID:Addgene_30175 Copy   

•   Source: Addgene

http://www.addgene.org/31851

Species:
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria.

Proper citation: RRID:Addgene_31851 Copy   

•   Source: Addgene

http://www.addgene.org/31820

Species:
Genetic Insert: GFP (lacking ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria.

Proper citation: RRID:Addgene_31820 Copy   

•   Source: Addgene

http://www.addgene.org/32379

Species:
Genetic Insert: int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pMl603; Vector Types:Other, bacterial integration vector; Bacterial Resistance:Hygromycin
References:
Comments: This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference. Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.

Proper citation: RRID:Addgene_32379 Copy   

•   Source: Addgene

http://www.addgene.org/32378

Species:
Genetic Insert: int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:Other, bacterial integration vector; Bacterial Resistance:Hygromycin
References:
Comments: This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional interference.

Proper citation: RRID:Addgene_32378 Copy   

•   Source: Addgene

http://www.addgene.org/119885

Species:
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_119885 Copy   

•   Source: Addgene

http://www.addgene.org/17975

Species:
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:Other, mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379. pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+

Proper citation: RRID:Addgene_17975 Copy   

•   Source: Addgene

http://www.addgene.org/27057

Species: synthetic gene
Genetic Insert: P#38-gfp-DAS+4
Vector Backbone Description: Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP.

Proper citation: RRID:Addgene_27057 Copy   

•   Source: Addgene

http://www.addgene.org/117695

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli. This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA. References: this paper has been accepted in RSC Advances: A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam

Proper citation: RRID:Addgene_117695 Copy   

•   Source: Addgene

http://www.addgene.org/204626

Species: Homo sapiens
Genetic Insert: HAPPID1 heavy chain
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6441; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_204626 Copy   

•   Source: Addgene

http://www.addgene.org/118076

Species: Other
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_118076 Copy   

•   Source: Addgene

http://www.addgene.org/157876

Species:
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_157876 Copy   

•   Source: Addgene

http://www.addgene.org/219768

Species: Other
Genetic Insert: Pv4CL1
Vector Backbone Description: Backbone Size:4111; Vector Backbone:MoClo adapted pGAPZ-vector with hygromycin resistance; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_219768 Copy   

•   Source: Addgene

http://www.addgene.org/222353

Species: Other
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:p15A and pWH1266; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.

Proper citation: RRID:Addgene_222353 Copy   

•   Source: Addgene

http://www.addgene.org/222357

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Tn7 expression vector.

Proper citation: RRID:Addgene_222357 Copy   

•   Source: Addgene

http://www.addgene.org/227580

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9915; Vector Backbone:pBAC2015, pGM1190, and pUC19; Vector Types:Other; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_227580 Copy   

•   Source: Addgene

http://www.addgene.org/209018

Species: Other
Genetic Insert: Deficient in the P2 bacteriophage packaging cos site; rhamnose-inducible P4 delta (δ) and epsilon (ε) genes
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Hygromycin
References:
Comments: P2 bacteriophage lysogen derived from E. coli EMG C-5545 strain. We designed a pair of primers to check the presence of P2 lysogen in this strain: - P2 gpH C-ter gpG Fw: 5’ GCAGAGCACGAACAGTCACG 3’ - P2 gpH C-ter gpG Rv: 5’ TTATTGCGGCATTTCCGGCC 3’ These primers amplify the C-terminal region of the P2 gpH gene (tail fiber) and the complete gpG gene (tail fiber chaperone) (PCR fragment size: 2070 bp).

Proper citation: RRID:Addgene_209018 Copy   

•   Source: Addgene

http://www.addgene.org/232335

Species: Other
Genetic Insert: PbGH3
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_232335 Copy   

•   Source: Addgene

http://www.addgene.org/231247

Species: Other
Genetic Insert: PPE18-ALFA
Vector Backbone Description: Backbone Marker:Bryson Lab; Backbone Size:6512; Vector Backbone:pBB115; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:

Proper citation: RRID:Addgene_231247 Copy   

•   Source: Addgene