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Species: Mus musculus
Genetic Insert: Soma-localized ChrimsonR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124603 Copy
Species: Other
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: Homo sapiens
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:Other, PCR Template; Bacterial Resistance:Ampicillin
References:
Comments: Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint
Proper citation: RRID:Addgene_124790 Copy
Species: Homo sapiens
Genetic Insert: hTRIM28
Vector Backbone Description: Backbone Marker:Barton Lab; Vector Backbone:pCMX-(Flag)x2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124960 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM4D(Gi)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121538 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: The firefly luciferase vector was modified from pGL3 Control Vector (Promega), such that a short sequence containing multiple cloning sites (5'-AGCTCTATACGCGTCTCAAGCTTACTGCTAGC GT-3') was inserted into the XbaI site immediately downstream from the stop codon.
3'UTR segments of target genes can be inserted into this vector to test for their effects on mRNA stability.
Proper citation: RRID:Addgene_12178 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.
Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_12208 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12214 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12217 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Created by 3-way ligation of PCR products: B/Sac-Sac/Eco-Eco/B.
Pak1 may contain mutations G401S and L516I. Depositor states that this should not affect plasmid function.
Proper citation: RRID:Addgene_12211 Copy
Species: Homo sapiens
Genetic Insert: Pak1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has found that this plasmid is extremely slow growing. We recommend keeping plates and liquid cultures growing for at least 20 hours. Overnight will not be sufficient.
There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way.
Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_12212 Copy
Species: Synthetic
Genetic Insert: rsCaMPARI
Vector Backbone Description: Backbone Size:4270; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122092 Copy
Species:
Genetic Insert: N MYC
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12540 Copy
Species: Homo sapiens
Genetic Insert: Rad51
Vector Backbone Description: Backbone Size:3300; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125570 Copy
Species: Synthetic
Genetic Insert: SYK biosensor
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego; Backbone Size:5505; Vector Backbone:pcDNA3.1’; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125729 Copy
Species: Simian virus
Genetic Insert: SV40 Large T, HSV1-TK
Vector Backbone Description: Backbone Size:12290; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
Proper citation: RRID:Addgene_12246 Copy
Species: Homo sapiens
Genetic Insert: TERT, HSV1-TK
Vector Backbone Description: Backbone Size:13115; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12245 Copy
Species: Mus musculus
Genetic Insert: reelin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The entire reelin open reading frame was assembled by fusing five overlapping cDNA clones isolated previously (D’Arcangelo et al., 1995) and subcloning them into pcDNA3. The following reelin fragments were used: 1.2 kb NaeI–SalI from p59BS1, 1.3 kb SalI–NdeI from pBS2, 3.1 kb NdeI–BspEI from pBS6, 770 bp BspEI–ApaLI from p3Rea3, and 4.2 kb ApaLIEcoRV from pBS53. The final clone (pCrl) contains the entire reelin open reading frame (10,383 bp) plus 95 bp of sequence 59 to the initiator methionine codon and 82 bp of 39 untranslated sequence (reelin cDNA nucleotides 188–10,748).
Proper citation: RRID:Addgene_122443 Copy
Species:
Genetic Insert: iCre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122518 Copy
Species:
Genetic Insert: Rev
Vector Backbone Description: Backbone Size:4174; Vector Backbone:pRSV-Rev; Vector Types:Mammalian Expression, Lentiviral, Other, Packaging; Bacterial Resistance:Ampicillin
References:
Comments: Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.
Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
Proper citation: RRID:Addgene_12253 Copy
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