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Species:
Genetic Insert: phytoene-beta carotene synthase
Vector Backbone Description: Vector Backbone:LCB7; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: The minor differences between the sequence and .gb file are of no functional concern.
Proper citation: RRID:Addgene_140232 Copy
Species:
Genetic Insert: dCas9 & Csy4
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section.
Proper citation: RRID:Addgene_140664 Copy
Species:
Genetic Insert: dCas9, LuxR & Csy4
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section.
Proper citation: RRID:Addgene_140665 Copy
Species: Other
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Other, Transposon; Bacterial Resistance:Spectinomycin
References:
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140625 Copy
Species: Other
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-tra13
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Other, Transposon; Bacterial Resistance:Spectinomycin
References:
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140628 Copy
Species: Other
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Other, Transposon; Bacterial Resistance:Spectinomycin
References:
Comments: An unexpected 65 bp insertion was detected in the origin of replication, compared to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140622 Copy
Species: Other
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression, CRISPR, Other, Transposon; Bacterial Resistance:Spectinomycin
References:
Comments: An unexpected 65 bp insertion was detected in the origin of replication, comparing to the sequence of the backbone plasmid pCDFDuet-1. But, the plasmid has been proven to work normally.
Proper citation: RRID:Addgene_140624 Copy
Species:
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the pcaHG genes in ADP1.
Proper citation: RRID:Addgene_140637 Copy
Species:
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:CRISPRi-ME (Jeffrey Barrick Laboratory); Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Derivative of CRISPRi-ME from Peng et al., "Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure", ACS Synthetic Biology, 2019. Vector was modified to take the dCas9 to Cas9 and gRNA was substituted to no longer target the insertion sequences, but instead the vanAB genes in ADP1.
Proper citation: RRID:Addgene_140638 Copy
Species: Other
Genetic Insert: Mb2Cas12a RVRR variant
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Other, Gateway compatible Cas12a entry clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_160327 Copy
Species: Other
Genetic Insert: Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160640 Copy
Species: Synthetic
Genetic Insert: GRLacIBDGal4AD / RDF
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160642 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Pasin et al., ACS Synth Biol. 2017;6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354; Backbone Size:3349; Vector Backbone:pLX-B3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: GoldenBraid compatibility: enzyme in BsmBI, enzyme out BsaI.
Proper citation: RRID:Addgene_160643 Copy
Species: Synthetic
Genetic Insert: BASTA / Cas9
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Compatible with GoldenBraid; insert can be released with BsaI.
Proper citation: RRID:Addgene_160647 Copy
Species: Other
Genetic Insert: Formaldehyde dehydrogenase promoter
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_160914 Copy
Species: Other
Genetic Insert: Dihydroxyacetone synthase 1 promoter
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_160916 Copy
Species: Other
Genetic Insert: Mitochondrial aldehyde dehydrogenase promoter
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_160919 Copy
Species: Synthetic
Genetic Insert: RUBY and At2S3 promoter
Vector Backbone Description: Backbone Size:9346; Vector Backbone:pHDE; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_160906 Copy
Species: Other
Genetic Insert: Formate dehydrogenase promoter
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_160921 Copy
Species: Other
Genetic Insert: Methanol oxidase promoter
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.23.424034v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_160922 Copy
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