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Species: Homo sapiens
Genetic Insert: RAGE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23645 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193187 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193185 Copy
Species: Homo sapiens
Genetic Insert: ANLN homology arms with mNeonGreen-linker
Vector Backbone Description: Backbone Marker:Thermo Fischer Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Mammalian Expression, CRISPR, Other, Donor template; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183834 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193174 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193188 Copy
Species: Homo sapiens
Genetic Insert: AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion
Vector Backbone Description: Backbone Marker:Thermo Fischer Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Mammalian Expression, CRISPR, Other, Donor template; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183871 Copy
Species: Homo sapiens
Genetic Insert: RHOA sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9-mRuby2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183877 Copy
Species: Homo sapiens
Genetic Insert: RHOA sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183878 Copy
Species: Homo sapiens
Genetic Insert: ANLN sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183874 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9-mRuby2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183888 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183889 Copy
Species: Homo sapiens
Genetic Insert: MYH10 sgRNA spacer
Vector Backbone Description: Vector Backbone:pX459V2.0-HypaCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183887 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193146 Copy
Species: Homo sapiens
Genetic Insert: MYH10 homology arms with mRuby2-linker
Vector Backbone Description: Backbone Marker:Thermo Fischer Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Mammalian Expression, CRISPR, Other, Donor template; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183869 Copy
Species: Homo sapiens
Genetic Insert: PXK-PX (1-125)
Vector Backbone Description: Vector Backbone:pGEX4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Amino Acid Sequence: MAFMEKPPAGKVLLDDTVPLTAAIEASQSLQSHTEYIIRVQRGISVENSWQIVRRYSDFDLLNNSLQIAGLSLPLPPKKLIGNMDREFIAERQKGLQNYLNVITTNHILSNCELVKKFLDPNNYS
Proper citation: RRID:Addgene_119114 Copy
Species: Homo sapiens
Genetic Insert: PXK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23723 Copy
Species: Homo sapiens
Genetic Insert: UBASH3A
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_192095 Copy
Species: Homo sapiens
Genetic Insert: UBASH3A
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_192099 Copy
Species: Homo sapiens
Genetic Insert: UBASH3A
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_192098 Copy
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