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Species: Synthetic
Genetic Insert: RNF2 +41 ngRNA
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lentiCRISPRv2_deltaCas9; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_207358 Copy
Species: Synthetic
Genetic Insert: L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_207357 Copy
Species: Synthetic
Genetic Insert: L227R-CFTR +15 ngRNA
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lentiCRISPRv2_deltaCas9; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_207360 Copy
Species: Homo sapiens
Genetic Insert: GFP P2A RFP_b-globin intron x2
Vector Backbone Description: Vector Backbone:SFFV-tet3G lentiviral backbone; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_216221 Copy
Species: Other
Genetic Insert: MutY (adenine glycosylase)
Vector Backbone Description: Backbone Size:4557; Vector Backbone:pkk223; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.04.05.535768v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_213110 Copy
Species: Homo sapiens
Genetic Insert: GFP P2A RFP_stop
Vector Backbone Description: Vector Backbone:SFFV-tet3G lentiviral backbone; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_216223 Copy
Species:
Genetic Insert: mtagBFP-KASH
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2023.09.18.558077 for bioRxiv preprint.
Proper citation: RRID:Addgene_213480 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Darrell Kotton; Backbone Size:7674; Vector Backbone:p2701-AGER-tdTomato; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.19.524655v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216474 Copy
Species: Synthetic
Genetic Insert: EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Vector Backbone:lentiCas9-Blast (Plasmid #52962); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_216130 Copy
Species: Synthetic
Genetic Insert: AsDR-filler-AsDR8, GFP-neo
Vector Backbone Description: Vector Backbone:pRG212; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Digest with BsmBI to replace filler with sgRNA oligos.
Please visit https://doi.org/10.1101/2023.09.18.558350 for bioRxiv preprint.
Proper citation: RRID:Addgene_217338 Copy
Species: Synthetic
Genetic Insert: crNT-3
Vector Backbone Description: Vector Backbone:pCH49; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2023.09.18.558350 for bioRxiv preprint.
Proper citation: RRID:Addgene_217339 Copy
Species: Synthetic
Genetic Insert: PP7 coat protein 3x Halo-GB1
Vector Backbone Description: Backbone Marker:in house; Backbone Size:5891; Vector Backbone:L50; Vector Types:Mammalian Expression, Other, PiggyBac; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_198337 Copy
Species: Other
Genetic Insert: denAsCas12a
Vector Backbone Description: Backbone Marker:Marco Jost, Jonathan Weissman; Vector Backbone:Addgene plasmid # 188765; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2023.09.18.558350 for bioRxiv preprint.
Proper citation: RRID:Addgene_217331 Copy
Species: Synthetic
Genetic Insert: sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
Vector Backbone Description: Vector Backbone:pMC.BESPX; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
References:
Comments: It is neccesary to remove plasmid backbone with minicircle technology (https://www.systembio.com/products/gene-expression-systems/minicircle-technology).
Proper citation: RRID:Addgene_216125 Copy
Species: Synthetic
Genetic Insert: sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
Vector Backbone Description: Vector Backbone:pMC.BESPX; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
References:
Comments: It is neccesary to remove plasmid backbone with minicircle technology (https://www.systembio.com/products/gene-expression-systems/minicircle-technology).
Proper citation: RRID:Addgene_216124 Copy
Species: Homo sapiens
Genetic Insert: NAP1
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_217610 Copy
Species:
Genetic Insert: (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
Vector Backbone Description: Vector Backbone:pAAV.CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.08.24.554624v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_209723 Copy
Species: Synthetic
Genetic Insert: hU6-crNT-EFS-PuroR-WPRE
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_214876 Copy
Species: Synthetic
Genetic Insert: hU6-crSURF1-EFS-PuroR-WPRE
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: RfxCas13d guide array targeting LAG3, FAS, CTLA4, PDCD1 (PD-1), HAVCR2 (TIM3)
Proper citation: RRID:Addgene_214878 Copy
Species: Synthetic
Genetic Insert: EF1a-mAmetrine-CAAX-WPRE
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Vector Backbone:lentiCas9-Blast (Plasmid #52962); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_216132 Copy
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