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Species: Saccharomyces cerevisiae
Genetic Insert: FLP1
Vector Backbone Description: Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and
CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids.
Proper citation: RRID:Addgene_160380 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SKI1
Vector Backbone Description: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160382 Copy
Species: Synthetic
Genetic Insert: hM4D(Gi) (partial )
Vector Backbone Description: Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_180787 Copy
Species: Synthetic
Genetic Insert: ChrimsonR
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192587 Copy
Species: Synthetic
Genetic Insert: eOPN3
Vector Backbone Description: Backbone Size:4098; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192596 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_190736 Copy
Species: Synthetic
Genetic Insert: Aion
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192577 Copy
Species: Homo sapiens
Genetic Insert: mCherry-Rheb(C181S)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192437 Copy
Species: Mus musculus
Genetic Insert: Gsdmd
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_80950 Copy
Species: Mus musculus
Genetic Insert: Lef1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: From Gabrielle Kardon.
Proper citation: RRID:Addgene_14021 Copy
Species: Homo sapiens
Genetic Insert: mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS
Vector Backbone Description: Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192445 Copy
Species: Homo sapiens
Genetic Insert: truncated ADAM metallopeptidase domain 10
Vector Backbone Description: Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains a Y316H mutation in hADAM10 compared to the NCBI reference sequence NP_001101.1. This mutation was present in the original DNA that was was PCR amplified from human placenta cDNA and is not known to affect plasmid function.
Proper citation: RRID:Addgene_189555 Copy
Species: Mus musculus
Genetic Insert: Gasdermin D
Vector Backbone Description: Vector Backbone:pCAG-FOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Backbone is from Dr. Eiji Morita
Proper citation: RRID:Addgene_111565 Copy
Species: Homo sapiens
Genetic Insert: truncated secreted frizzled related protein 1
Vector Backbone Description: Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_189550 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73704 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73703 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73702 Copy
Species: Mus musculus
Genetic Insert: RIPK3
Vector Backbone Description: Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73705 Copy
Species: Homo sapiens
Genetic Insert: mCherry-TSC2
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192440 Copy
Species: Other
Genetic Insert: Methyl coenzyme M reductase
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192767 Copy
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