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Species: Synthetic
Genetic Insert: Y-TAGA-Evo1 qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134808 Copy
Species: Other
Genetic Insert: wtCas9
Vector Backbone Description: Backbone Size:1718; Vector Backbone:pcdf1-b; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://doi.org/10.1101/2020.06.13.149682 for bioRxiv preprint.
Proper citation: RRID:Addgene_157913 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187513 Copy
Species: Synthetic
Genetic Insert: 3xStop
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187512 Copy
Species: Synthetic
Genetic Insert: OriT
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187510 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187507 Copy
Species: Synthetic
Genetic Insert: MitoTP
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187505 Copy
Species: Synthetic
Genetic Insert: amilCP
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187504 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187517 Copy
Species: Synthetic
Genetic Insert: t49202
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187521 Copy
Species: Synthetic
Genetic Insert: B0015
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187520 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187527 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCA; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187539 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCA; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187537 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCA; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187536 Copy
Species: Synthetic
Genetic Insert: peroxTP
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187515 Copy
Species: Synthetic
Genetic Insert: J23101
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187488 Copy
Species: Synthetic
Genetic Insert: pAOX1
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187490 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene plasmid 46881; Backbone Size:6733; Vector Backbone:pDL276; Vector Types:Bacterial Expression, Other, E. coli/Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Spectinomycin
References:
Comments: This plasmid may contain unstable elements. Prolonged growth in E. coli selects for deletions, but not in E. faecalis. Test multiple clones upon to ensure the full plasmid is selected.
Proper citation: RRID:Addgene_46882 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCO; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_187547 Copy
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