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Species: Homo sapiens
Genetic Insert: PIK3CG
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments: a972g, t981c, c2025t silent mutations not noted in Refseq
Proper citation: RRID:Addgene_70453 Copy
Species: Homo sapiens
Genetic Insert: PIK3CG
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments: a972g, t981c, c2025t silent mutations not noted in Refseq
Proper citation: RRID:Addgene_70454 Copy
Species: Homo sapiens
Genetic Insert: PIK3CG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81843 Copy
Species: Homo sapiens
Genetic Insert: PIK3CG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_81630 Copy
Species: Homo sapiens
Genetic Insert: SMAD3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert. Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82272 Copy
Species: Homo sapiens
Genetic Insert: YAP1 WT
Vector Backbone Description: Vector Backbone:pDONR; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_184524 Copy
Species: Homo sapiens
Genetic Insert: SNF1LK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23838 Copy
Species: Other
Genetic Insert: TpenDE Pc21g21370 terminator
Vector Backbone Description: Vector Backbone:pICH41276; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_171349 Copy
Species: Synthetic
Genetic Insert: DR-SMN2-2-DR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118653 Copy
Species: Synthetic
Genetic Insert: dCasRx
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118656 Copy
Species: Synthetic
Genetic Insert: SMN2-DN-RG6-SA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118655 Copy
Species: Synthetic
Genetic Insert: gRG6-SA
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118652 Copy
Species: Synthetic
Genetic Insert: gCasRx-CUG
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118645 Copy
Species: Synthetic
Genetic Insert: gSMN2-DN2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118647 Copy
Species: Synthetic
Genetic Insert: gSMN2-DN1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
Proper citation: RRID:Addgene_118646 Copy
Species: Homo sapiens
Genetic Insert: LIG1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_83006 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139134 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139137 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139138 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:CloCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/529180v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139135 Copy
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