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  • Organism:rattus norvegicus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGEX-parkin W403A-C431S
 
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RRID:Addgene_45977 Parkin W403A-C431S Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Double mutation W403A-C431S 2022-04-22 03:41:31 0
pGEX-parkin 219-465
 
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RRID:Addgene_45972 Parkin 219-465 Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Deleted a.a. 1-219 2022-04-22 03:41:31 0
pGEX-parkin F146A
 
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RRID:Addgene_45973 Parkin F146A Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Changed Phe146 to alanine. 2022-04-22 03:41:31 0
pGEX-parkin F463A
 
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RRID:Addgene_45975 Parkin F463A Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression. Changed Phe463 to alanine. 2022-04-22 03:41:31 0
pGEX-parkin WT
 
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RRID:Addgene_45969 Parkin Rattus norvegicus Ampicillin PMID:23661642 Backbone Marker:Amersham; Backbone Size:4966; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Codon usage optimized for E.coli expression 2022-04-22 03:41:30 0
pXL-CAG-3XAP-NLG1
 
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RRID:Addgene_43923 3XAP-NLG1 Rattus norvegicus Ampicillin PMID:23457442 Backbone Size:6390; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:41:06 0
pMA3411
 
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RRID:Addgene_46877 soluble guanylate cyclase1 beta 3 Rattus norvegicus Ampicillin PMID:22637478 Backbone Size:5684; Vector Backbone:pMA1662; Vector Types:Retroviral; Bacterial Resistance:Ampicillin E473K, C541D 2022-04-22 03:41:47 0
Flag-GLK
 
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RRID:Addgene_47402 MAP4K3 Rattus norvegicus Ampicillin PMID:11384986 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:41:58 0
pRc/CMV HA TrkB-T1
 
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RRID:Addgene_39980 TrkB.T1 Rattus norvegicus Ampicillin PMID:9927421 The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI. See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant. Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin TrkB.T1 variant; endogenous signal sequence was replaced with BM40 and HA tag 2022-04-22 03:40:01 1
pRc/CMV HA TrkC
 
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RRID:Addgene_39979 TrkC Rattus norvegicus Ampicillin PMID:9927421 cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkC was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequence for GenBank ID NM_001270656.1, the TrkC protein sequence starts at amino acid #32 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin endogenous signal sequence was replaced with BM40 and HA tag 2022-04-22 03:40:01 0
pRc/CMV HA TrkA
 
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RRID:Addgene_39977 TrkA Rattus norvegicus Ampicillin PMID:9927421 cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRC/CMV AC7 vector, a derivative of the pRC/CMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin endogenous signal sequence was replaced with BM40 and HA tag 2022-04-22 03:40:01 0
pCMV Myc rat SAPAP4
 
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RRID:Addgene_40218 SAPAP4 Rattus norvegicus Ampicillin PMID:9115257 Vector Backbone:pCMV5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N834D 2022-04-22 03:40:04 0
pCMV Myc rat SAPAP2
 
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RRID:Addgene_40216 SAPAP2 Rattus norvegicus Ampicillin PMID:9115257 Vector Backbone:pCMV5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin H137Q and K806R 2022-04-22 03:40:04 0
pcDNA3 βarr1 S412D Flag
 
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RRID:Addgene_42196 β-arrestin 1 S412D Rattus norvegicus Ampicillin PMID:9924018 Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing. Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S412D 2022-04-22 03:40:37 0
pCMV5-myc-Erk2
 
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1+ mentions
RRID:Addgene_39222 Erk2 Rattus norvegicus Ampicillin PMID:11352917 Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:39:51 1
pRSET-RCaMP1h
 
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RRID:Addgene_42874 Red Fluorescent Calcium binding Protein Rattus norvegicus Ampicillin PMID:23459413 Gene was cloned using BaMHI and HindIII - But part of the vector backbone then becomes part of the protein (leader sequence containing histag) - using NdeI and HindIII will completely remove the open reading frame/gene. Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2753; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:40:53 3
pcDNA3-AU1-mTOR-E2419K
 
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RRID:Addgene_19994 mammalian target of rapamycin-E2419K Rattus norvegicus Ampicillin PMID:17360675 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Changed Glutamine 2419 to Lysine 2022-04-22 03:35:36 0
RSV CREB
 
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RRID:Addgene_22394 CREB Rattus norvegicus Ampicillin PMID:2573431 insert can be released by digesting with HindIII/Xba1 Backbone Size:3600; Vector Backbone:RSV-SG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:36:01 1
RSV CREB M1
 
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RRID:Addgene_22395 CREB Rattus norvegicus Ampicillin PMID:2573431 Backbone Size:3600; Vector Backbone:RSV-SG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Changed Serine 133 to Alanine 2022-04-22 03:36:01 0
pR-BAM
 
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RRID:Addgene_22649 Myelin Transcription Factor 1L Rattus norvegicus Ampicillin PMID:9373037 Used to make riboprobes by cutting with SmaI and transcribing with SP6 polymerase to give a 980 bp riboprobe for RNA analysis on Northern blots This Rat Myt1 DNA cloned by Hudson lab. Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Other, Cloning and RNA Synthesis; Bacterial Resistance:Ampicillin 2022-04-22 03:36:06 0

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