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Species:
Genetic Insert: Opto-T7RNAP*(563)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101661 Copy
Species:
Genetic Insert: Opto-T7RNAP*(563), half expr.
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101667 Copy
Species:
Genetic Insert: Opto-T7RNAP*(302)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101662 Copy
Species:
Genetic Insert: Opto-T7RNAP*(600)
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_101664 Copy
Species: Synthetic
Genetic Insert: EcoRNAPα-PmCDA1
Vector Backbone Description: Backbone Size:5023; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_189824 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERV1
Vector Backbone Description: Vector Backbone:pDisCoTune; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_173713 Copy
Species: Synthetic
Genetic Insert: mNeonGreen; tetR
Vector Backbone Description: Backbone Size:1706; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_181944 Copy
Species: Synthetic
Genetic Insert: mNG-TorR; mCherry; tetR
Vector Backbone Description: Backbone Size:1706; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_181947 Copy
Species: Other
Genetic Insert: RK2/RP4 origin of transfer
Vector Backbone Description: Backbone Marker:Slattery et al., 2018; Backbone Size:9507; Vector Backbone:pPtGE31; Vector Types:Synthetic Biology, Other, Bacterial and Yeast Cloning; Conjugation; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_165986 Copy
Species: Other
Genetic Insert: Cas13b nuclease from Porphyromonas gingivalis
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD33; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_184833 Copy
Species: Other
Genetic Insert: Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
Vector Backbone Description: Backbone Size:4258; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_184834 Copy
Species: Other
Genetic Insert: Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
Vector Backbone Description: Backbone Size:4258; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_184835 Copy
Species:
Genetic Insert: SpCas9 gRNA scaffold + Csy4 sequence
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_182827 Copy
Species:
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pAM2314; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_165582 Copy
Species:
Genetic Insert: Cas12c single guide RNA targeting GFP
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_183070 Copy
Species: Synthetic
Genetic Insert: Cas12c tracrRNA and pre-crRNA
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments: A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
Proper citation: RRID:Addgene_183076 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_183222 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_114252 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXBC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_114251 Copy
Species: Synthetic
Genetic Insert: LacZalpha
Vector Backbone Description: Vector Backbone:pMXLC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_114222 Copy
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