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Species: Saccharomyces cerevisiae
Genetic Insert: D10A-GCN4
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113025 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MS2 bacteriophage coat protein
Vector Backbone Description: Vector Backbone:pRS315; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113394 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3-his3AI
Vector Backbone Description: Vector Backbone:YCplac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113393 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ty3 Gag3 codon optimized for E. coli expression
Vector Backbone Description: Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_113397 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Red fluorescent protein, codon optimized for S. cerevisiae
Vector Backbone Description: Vector Backbone:pYES2.0; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_113396 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5000; Vector Backbone:TMBS (CEN TRP1 MET3 promoter); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC12
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: 7055
Proper citation: RRID:Addgene_1163 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC12
Vector Backbone Description: Backbone Size:5554; Vector Backbone:pRS316:GAL1-10; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: 7058
Proper citation: RRID:Addgene_1166 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC12
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 11R Unique
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:3956; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_174830 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OsTIR1(WT)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_140655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74G)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_140656 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pRS306-GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_140657 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mCherry-CaaX
Vector Backbone Description: Backbone Size:1811; Vector Backbone:pGT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139500 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139487 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pHSP12 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pGRE2 24xPP7sl
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pHIS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pIME2
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Other, Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pSAE3
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Other, Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191787 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1 pCUP1 with 2 phlO sites
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Other, Yeast genomic targeting; Bacterial Resistance:Ampicillin
References:
Comments: pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University.
New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands
Shigehito Ikushima and Jef D. Boeke
ACS Synthetic Biology 2017 6 (3), 497-506
DOI: 10.1021/acssynbio.6b00205
Proper citation: RRID:Addgene_191794 Copy
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