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Species: Rattus norvegicus
Genetic Insert: beta-arrestin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_14685 Copy
Species: Rattus norvegicus
Genetic Insert: beta arrestin 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_14693 Copy
Species: Rattus norvegicus
Genetic Insert: Glp1-R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_14944 Copy
Species: Rattus norvegicus
Genetic Insert: ERK1
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15006 Copy
Species: Rattus norvegicus
Genetic Insert: p130cas
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15001 Copy
Species: Rattus norvegicus
Genetic Insert: Zdk2, Cofilin, LOV2
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_141136 Copy
Species: Rattus norvegicus
Genetic Insert: Zdk2, Cofilin, LOV2
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_141137 Copy
Species: Rattus norvegicus
Genetic Insert: PV-DsRed-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pcDNA5/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16341 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES-DsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5700; Vector Backbone:pcDNA5/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note- this plasmid may contain the NES N-terminal to the PV insert, not C-terminal as the plasmid name suggests. Also, there is a Myc tag at the C-terminus of the DsRed.
Proper citation: RRID:Addgene_16339 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences. An additional C-terminal mutation, H579Y, is present. These mutations are not believed to affect function.
Proper citation: RRID:Addgene_16379 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences and are therefore not believed to affect function.
Proper citation: RRID:Addgene_16378 Copy
Species: Rattus norvegicus
Genetic Insert: PKC beta 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16380 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP5G
Vector Backbone Description: Vector Backbone:pEGFP-N1 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_162519 Copy
Species: Rattus norvegicus
Genetic Insert: Brain derived neurotrophic factor
Vector Backbone Description: Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_162718 Copy
Species: Rattus norvegicus
Genetic Insert: Myosin Va
Vector Backbone Description: Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_162715 Copy
Species: Rattus norvegicus
Genetic Insert: rat Δ188Annexin A11
Vector Backbone Description: Backbone Marker:Gunter Stier; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_164497 Copy
Species: Rattus norvegicus
Genetic Insert: rat Δ192Annexin A11
Vector Backbone Description: Backbone Marker:Gunter Stier; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_164498 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1-HA-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Comments: Rfz1 from Henk Roelink. This sequence has a few differences.
from the published sequence. Sites listed in Rfz1 are not a complete list.
and I haven't searched through the CS2+ sequence to see if/where those sites.
are present. HA tag version made by Jeff Brown (2/98-3/98) insert the epitope.
into the StyI site (1904). The 3' Sty site is destroyed by the tags, but.
the 5' site is inteact. Epitope can be detected in PBS/Formaldehyde fixed.
tissue permeablized with 0.2% Triton. Frizzled tends to ppt out of SDS sample.
buffer when boiled, so either warm samples to ~70 degrees Celsius for a.
few minutes before loading samples or use a strong denaturing loading buffer.
and incubate the samples at room temp for half and hour before loading.
The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16797 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: The original rat Frizzled-2 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The the insert was published by Wang et al., JBC 271: 4468 (1996), and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16813 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Other, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: The original rat Frizzled-2 cDNA, tagged with a single epitope in the third extracellular loop (at an introduced NheI site) was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The myc-tagged insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16812 Copy
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