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Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23888 Copy
Species: Other
Genetic Insert: OsD27
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_178285 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_70415 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_70416 Copy
Species: Homo sapiens
Genetic Insert: FBXW7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81502 Copy
Species: Homo sapiens
Genetic Insert: FBXW7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81576 Copy
Species: Homo sapiens
Genetic Insert: FBXW7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_81973 Copy
Species: Homo sapiens
Genetic Insert: FBXW7
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: CLOSED (stop codon immediately follows insert)
Proper citation: RRID:Addgene_82780 Copy
Species: Homo sapiens
Genetic Insert: FBXW7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Open state: NO stop codon immediately follows insert
Proper citation: RRID:Addgene_81494 Copy
Species: Mus musculus
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, TOPO TA cloning vector; Bacterial Resistance:Spectinomycin
References:
Comments: Insert contains Kozak sequence and no stop codon
Proper citation: RRID:Addgene_98618 Copy
Species:
Genetic Insert: chPheRS2 for DOPA
Vector Backbone Description: Backbone Size:4067; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_182257 Copy
Species: Synthetic
Genetic Insert: M.XbaI
Vector Backbone Description: Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_170767 Copy
Species: Synthetic
Genetic Insert: M.XhoI
Vector Backbone Description: Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_170768 Copy
Species: Homo sapiens
Genetic Insert: CD3E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81865 Copy
Species: Other
Genetic Insert: CcmO-PDT
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_165584 Copy
Species: Homo sapiens
Genetic Insert: CD3E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81314 Copy
Species: Homo sapiens
Genetic Insert: BAF47
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_111181 Copy
Species: Synthetic
Genetic Insert: Taz
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_104333 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency.
Proper citation: RRID:Addgene_104438 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtUBC19
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: release insert with BsaI and clone to Level 1 cloning vector
Proper citation: RRID:Addgene_104684 Copy
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