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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDONR223-ERBB2 Resource Report Resource Website |
RRID:Addgene_23888 | ERBB2 | Homo sapiens | Spectinomycin | PMID:21107320 | The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately. | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin | 2022-08-24 01:02:41 | 0 | |
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pCDFDuet-trAtCCD7-OsD27(pYL726 ) Resource Report Resource Website |
RRID:Addgene_178285 | OsD27 | Other | Spectinomycin | PMID:34533983 | Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-09-08 01:02:21 | 0 | ||
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R777-E131 Hs.MET Resource Report Resource Website |
RRID:Addgene_70415 | MET | Homo sapiens | Spectinomycin | PMID: | Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin | 2022-09-14 01:04:02 | 0 | ||
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R777-E132 Hs.MET-nostop Resource Report Resource Website |
RRID:Addgene_70416 | MET | Homo sapiens | Spectinomycin | PMID: | Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin | 2022-09-14 01:04:02 | 0 | ||
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pDONR223_FBXW7_p.R658Q Resource Report Resource Website |
RRID:Addgene_81502 | FBXW7 | Homo sapiens | Spectinomycin | PMID:27147599 | Closed state: stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | R658Q | 2022-09-10 01:04:24 | 0 |
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pDONR223_FBXW7_p.I347M Resource Report Resource Website |
RRID:Addgene_81576 | FBXW7 | Homo sapiens | Spectinomycin | PMID:27147599 | Closed state: stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | I347M | 2022-09-10 01:04:24 | 0 |
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pDONR223_FBXW7_WT Resource Report Resource Website |
RRID:Addgene_81973 | FBXW7 | Homo sapiens | Spectinomycin | PMID:27147599 | Open state: NO stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | WT | 2022-09-10 01:04:25 | 0 |
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pDONR223_FBXW7_p.P620R Resource Report Resource Website |
RRID:Addgene_82780 | FBXW7 | Homo sapiens | Spectinomycin | PMID:27478040 | CLOSED (stop codon immediately follows insert) | Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | P620R | 2022-09-10 01:04:26 | 0 |
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pDONR223_FBXW7_p.A502V Resource Report Resource Website |
RRID:Addgene_81494 | FBXW7 | Homo sapiens | Spectinomycin | PMID:27147599 | Open state: NO stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | A502V | 2022-09-10 01:04:24 | 0 |
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pCR8/GW/TOPO-SRF Resource Report Resource Website |
RRID:Addgene_98618 | SRF | Mus musculus | Spectinomycin | PMID:26146939 | Insert contains Kozak sequence and no stop codon | Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Other, TOPO TA cloning vector; Bacterial Resistance:Spectinomycin | 2022-09-13 01:04:39 | 0 | |
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pUltra-chPheRS Resource Report Resource Website |
RRID:Addgene_182257 | chPheRS2 for DOPA | Spectinomycin | PMID:34942167 | Backbone Size:4067; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-10-07 04:51:29 | 0 | |||
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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA Resource Report Resource Website |
RRID:Addgene_170767 | M.XbaI | Synthetic | Spectinomycin | PMID:35255734 | Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-10-14 01:18:36 | 0 | ||
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Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA Resource Report Resource Website |
RRID:Addgene_170768 | M.XhoI | Synthetic | Spectinomycin | PMID:35255734 | Backbone Marker:EMD Millipore/Novagen; Backbone Size:1724; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-10-14 01:03:09 | 0 | ||
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pDONR223_CD3E_WT Resource Report Resource Website |
RRID:Addgene_81865 | CD3E | Homo sapiens | Spectinomycin | PMID:27147599 | Closed state: stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | WT | 2022-11-23 12:04:58 | 0 |
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pCcmO-StrepII-PDT Resource Report Resource Website |
RRID:Addgene_165584 | CcmO-PDT | Other | Spectinomycin | PMID:34232633 | Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-11-23 12:02:14 | 0 | ||
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pDONR223_CD3E_p.E189Q Resource Report Resource Website |
RRID:Addgene_81314 | CD3E | Homo sapiens | Spectinomycin | PMID:27147599 | Closed state: stop codon immediately follows insert | Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin | E189Q | 2022-11-23 12:04:56 | 0 |
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pDONR SMARCB1 var 2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_111181 | BAF47 | Homo sapiens | Spectinomycin | PMID:30860482 | Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:17:40 | 1 | ||
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pBL132 Resource Report Resource Website |
RRID:Addgene_104333 | Taz | Synthetic | Spectinomycin | PMID:29650958 | Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2022-04-22 03:15:45 | 0 | ||
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pRW004-SM-destination-proUBQ10-Cas9 Resource Report Resource Website |
RRID:Addgene_104438 | Spectinomycin | PMID:30083222 | There are two additional BsaI cloning sites in the Cas9 sequences, but they should not affect the cloning process, since GoldenGate cloning can assemble four or more fragments into a backbone. The additional BsaI sites create four overhangs that are very different from the overhangs for integrating the sgRNAs, and there should be no major issues with fragment assembly or cloning efficiency. | Backbone Marker:Lampropoulos et al; Backbone Size:4114; Vector Backbone:pZ001; Vector Types:Bacterial Expression, Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2022-04-22 03:15:46 | 0 | |||
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pUG-UBC19 Resource Report Resource Website |
RRID:Addgene_104684 | AtUBC19 | Arabidopsis thaliana | Spectinomycin | PMID:29194629 | release insert with BsaI and clone to Level 1 cloning vector | Backbone Marker:self-made; Backbone Size:2251; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BpiI/BsaI sites removed by point-mutation | 2022-04-22 03:15:49 | 0 |
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