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Species: Synthetic
Genetic Insert: AbR Down Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119616 Copy
Species:
Genetic Insert: exo, beta, gam, sgRNA-FRT
Vector Backbone Description: Backbone Marker:Prather lab; Backbone Size:6959; Vector Backbone:pKDsgRNA-ack; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments: Must be used in combination with plasmid pCas9-CR4 (Addgene plasmid # 62655) to express Cas9.
Proper citation: RRID:Addgene_113398 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116012 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116006 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116007 Copy
Species: Arabidopsis thaliana
Genetic Insert: pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_116009 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_115998 Copy
Species: Bacteriophage lambda
Genetic Insert: Red-alpha, Red-beta, Gam
Vector Backbone Description: Backbone Size:0; Vector Backbone:F'; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: pML300 carries the genes encoding the exo, beta, and gam recombination functions of bacteriophage lambda, as well as RecA, to render an E. coli strain recombination proficient. The lambda Red-alpha, Red-beta, and Gam proteins are under the rhaB promoter, induced by incubation with the sugar rhamnose. The bacterial strain is BW28705I (BUN21)/pML300 (recipient strain): lacIq rrnB3 delta-lacZ4787 hsdR514 delta-(araBAD)567 delta-(rhaBAD)568 galU95 delta-endA9::FRT delta-recA635::FRT umuC::ParaBAD-I-SceI-FRT + pML300(PrhaB-gam-beta-exo Ts(ori). Grow at 30'C in LB/Sp 50 ug/ml (spectinomycin resistant).
Proper citation: RRID:Addgene_11675 Copy
Species: Other
Genetic Insert: EcAvs2
Vector Backbone Description: Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_188804 Copy
Species: Other
Genetic Insert: SeAvs3
Vector Backbone Description: Backbone Size:3423; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_188805 Copy
Species:
Genetic Insert: His-Ub
Vector Backbone Description: Vector Backbone:pSUMO1; Vector Types:; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_185756 Copy
Species: Other
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_190243 Copy
Species: Other
Genetic Insert: Retron Eco1 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187202 Copy
Species: Synthetic
Genetic Insert: er-mCherry
Vector Backbone Description: Backbone Size:11852; Vector Backbone:pMpGWB402; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_186288 Copy
Species: Mus musculus
Genetic Insert: Ddx58
Vector Backbone Description: Vector Backbone:pENTR223.1 backbone; Vector Types:Mammalian Expression, Other, Gateway entry vector; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_160107 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: CLOSED (stop codon immediately follows insert)
Proper citation: RRID:Addgene_82859 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Size:2693; Vector Backbone:pDonor-255; Vector Types:Other, Gateway Entry Clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_70346 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert Supporting reference: Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife. 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521
Proper citation: RRID:Addgene_82113 Copy
Species: Homo sapiens
Genetic Insert: ERBB2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2790; Vector Backbone:pDONR223; Vector Types:Other, Gateway Entry vector; Bacterial Resistance:Spectinomycin
References:
Comments: Closed state: stop codon immediately follows insert
Proper citation: RRID:Addgene_81705 Copy
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