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URL: http://www.wormbase.org/db/get?name=WBStrain00062213
Proper Citation: RRID:WB-STRAIN:WBStrain00062213
Description: Caenorhabditis elegans with name juSi164 unc-119(ed3) III; wtfIs335. from WB.
Species: Caenorhabditis elegans
Synonyms: juSi164 unc-119(ed3) III; wtfIs335.
Notes: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs335 [5xQUAS+(delta)pes-10p::AI::eTsChR::unc-54 3'UTR + rab-3p::AI::QF+hGR::SL2::tagBFP::unc-54 3'UTR + rab-3p::his-24::tagRFP::unc-54 3'UTR + rab-3p::his-24::GCaMP6s::unc-54 3'UTR]. Keep plates covered to avoid unnecessary exposure to light. Pan-neuronal eTsChR on activation using dex treatment via QF+hGR. Worms also express calcium sensing fluorescence protein- GCaMP6s in nuclei of each neurons. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622.
Affected Gene: WBGene00006843(unc-119)
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Source: Integrated Animals
Source Database: WormBase (WB)