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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD02820
Description: Tetrahymena thermophila with name MTT-HA-PP50 #5 from TSC.
Species: Tetrahymena thermophila
Notes: Used Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. PP50 is Ttherm. 00865300 into Ttherm 00865290 sequence. Need 100ug/ml pm in medium to keep the tagged gene present. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: PPO1 (TTHERM_00865290, TTHERM_00865300)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center