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URL: https://sites.wustl.edu/tetrahymena/finding-strains/
Proper Citation: RRID:TSC_SD02146
Description: Tetrahymena thermophila with name GTU-TAP (4) from TSC.
Species: Tetrahymena thermophila
Notes: Rescue of GTU05XGTU06 knockout heterokaryons with pTTMG-HA TAP. GTU1 with HA+TAP tag into MTT1 locus. MTT1 promoter, grow with 0.2ug/ml CdCl in 1xSPP. 5’MTT1-GTU-HA-TAP-MTT1 3’ TAP tag from Eric Phzicky is: GTU-HA-6XHis-3Cprotease cleavage-S.Aureus protein A. Constuct pTTMG-HA used to rescue GTU5 xGTU06 KO heterokaryons. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Affected Gene: GTU1 (TTHERM_00079520)
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Source: Integrated Animals
Source Database: TSC, Tetrahymena Stock Center
